Please use this identifier to cite or link to this item:<322
Title: Retrospective study of Western blot profiles in immune sera of natural dengue virus infections
Authors: Se-Thoe, S.Y.
Ng, M.M.L. 
Ling, A.E.
Keywords: Immunogenic
Primary and secondary infection
Structural proteins
Issue Date: 1999
Citation: Se-Thoe, S.Y.,Ng, M.M.L.,Ling, A.E. (1999). Retrospective study of Western blot profiles in immune sera of natural dengue virus infections. Journal of Medical Virology 57 (3) : 322-330. ScholarBank@NUS Repository.<322
Abstract: The Western blot (WB) assay was used to determine dengue virus antibodies present in human immune sera arising from recent primary and secondary dengue virus infections in Singapore. Cell lysates of dengue-2 virus-infected C6/36 and Vero cells were used. Antibodies directed against structural proteins of dengue-2 virus including envelope (E, gp60/50), capsid-premembrane (C-PrM, gp35), and premembrane (PrM, gp20) were detected, with antibody against envelope protein being most dominant. Similar WB profiles were detected in both primary and secondary dengue virus infections. The reactivity rate of antibodies to dengue-2 virus proteins was higher in infected Vero cell lysate than in infected C6/36 cell lysate, with the exception of antibodies to nonstructural proteins of NS1 and NS3, which were detected predominantly in infected C6/36 cell lysate. More than 75% of 'normal' individuals (with no complaint of recent dengue virus infection) examined had low levels of dengue virus antibodies, but all presented with similar WB profiles as patients with recent dengue virus infections. This finding reflects a high seroprevalence of dengue virus infections and the long lasting nature of E, C-PrM, and PrM antibodies. Results from this study indicate that in natural dengue virus infections, native E, C-PrM, and PrM antigens of dengue virus are immunogenic and elicit long-lasting antibodies.
Source Title: Journal of Medical Virology
ISSN: 01466615
DOI: 10.1002/(SICI)1096-9071(199903)57:3<322
Appears in Collections:Staff Publications

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