Please use this identifier to cite or link to this item:
|Title:||Activation of corticotropin-releasing factor 2 receptor inhibits Purkinje neuron P-type calcium currents via Goα-dependent PKC epsilon pathway||Authors:||Tao, J.
|Keywords:||Corticotropin-releasing factor receptor
P-type calcium channels
|Issue Date:||Sep-2009||Citation:||Tao, J., Zhang, Y., Huang, H., Jiang, X. (2009-09). Activation of corticotropin-releasing factor 2 receptor inhibits Purkinje neuron P-type calcium currents via Goα-dependent PKC epsilon pathway. Cellular Signalling 21 (9) : 1436-1443. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cellsig.2009.05.002||Abstract:||Corticotropin-releasing factor (CRF) receptors have been demonstrated to be widely expressed in the central nervous system and in many peripheral tissues of mammalians. However, it is still unknown whether CRF receptors will function in cerebellar Purkinje neurons. In the present study, we investigated the expression profile of CRF receptors in rat cerebellum and identified a novel functional role of CRFR2 in modulating Purkinje neuron P-type Ca2+ currents (P-currents). We found that CRFR2α mRNA, but not CRFR1 and CRFR2β, was endogenously expressed in rat cerebellum. Activation of CRFR2 by UCN2 inhibited P-currents in a concentration-dependent manner (IC50 ~ 0.07 μM). This inhibitory effect was abolished by astressin2B, a CRFR2 antagonist, and was blocked by GDP-β-S, pertussis toxin, or a selective antibody raised against the Goα. Inhibition of phospholipase C (PLC) blocked the inhibitory action of UCN2. The application of diacylglycerol (DAG) antagonist, 1-hexadecyl-2-acetyl-sn-glycerol, as well as inhibition of either protein kinase C or its epsilon isoform (PKCε) abolished the UCN2 effect while 1-oleoyl-2-acetyl-sn-glycerol (EI-150), a membrane-permeable DAG analogue, occluded UCN2-mediated inhibition. In addition, UCN2 significantly increases spontaneous firing frequency of Purkinje neurons in cerebellar slices. In summary, activation of CRFR2 inhibits P-currents in Purkinje neurons via Goα-dependent PLC/PKCε pathway, which might contribute to its physiological functions in the cerebellum. © 2009 Elsevier Inc. All rights reserved.||Source Title:||Cellular Signalling||URI:||http://scholarbank.nus.edu.sg/handle/10635/107460||ISSN:||08986568||DOI:||10.1016/j.cellsig.2009.05.002|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 25, 2020
WEB OF SCIENCETM
checked on Sep 25, 2020
checked on Sep 19, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.