Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cellsig.2009.05.002
DC FieldValue
dc.titleActivation of corticotropin-releasing factor 2 receptor inhibits Purkinje neuron P-type calcium currents via Goα-dependent PKC epsilon pathway
dc.contributor.authorTao, J.
dc.contributor.authorZhang, Y.
dc.contributor.authorHuang, H.
dc.contributor.authorJiang, X.
dc.date.accessioned2014-11-06T08:23:55Z
dc.date.available2014-11-06T08:23:55Z
dc.date.issued2009-09
dc.identifier.citationTao, J., Zhang, Y., Huang, H., Jiang, X. (2009-09). Activation of corticotropin-releasing factor 2 receptor inhibits Purkinje neuron P-type calcium currents via Goα-dependent PKC epsilon pathway. Cellular Signalling 21 (9) : 1436-1443. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cellsig.2009.05.002
dc.identifier.issn08986568
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/107460
dc.description.abstractCorticotropin-releasing factor (CRF) receptors have been demonstrated to be widely expressed in the central nervous system and in many peripheral tissues of mammalians. However, it is still unknown whether CRF receptors will function in cerebellar Purkinje neurons. In the present study, we investigated the expression profile of CRF receptors in rat cerebellum and identified a novel functional role of CRFR2 in modulating Purkinje neuron P-type Ca2+ currents (P-currents). We found that CRFR2α mRNA, but not CRFR1 and CRFR2β, was endogenously expressed in rat cerebellum. Activation of CRFR2 by UCN2 inhibited P-currents in a concentration-dependent manner (IC50 ~ 0.07 μM). This inhibitory effect was abolished by astressin2B, a CRFR2 antagonist, and was blocked by GDP-β-S, pertussis toxin, or a selective antibody raised against the Goα. Inhibition of phospholipase C (PLC) blocked the inhibitory action of UCN2. The application of diacylglycerol (DAG) antagonist, 1-hexadecyl-2-acetyl-sn-glycerol, as well as inhibition of either protein kinase C or its epsilon isoform (PKCε) abolished the UCN2 effect while 1-oleoyl-2-acetyl-sn-glycerol (EI-150), a membrane-permeable DAG analogue, occluded UCN2-mediated inhibition. In addition, UCN2 significantly increases spontaneous firing frequency of Purkinje neurons in cerebellar slices. In summary, activation of CRFR2 inhibits P-currents in Purkinje neurons via Goα-dependent PLC/PKCε pathway, which might contribute to its physiological functions in the cerebellum. © 2009 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.cellsig.2009.05.002
dc.sourceScopus
dc.subjectCorticotropin-releasing factor receptor
dc.subjectG-protein
dc.subjectP-type calcium channels
dc.subjectPurkinje neuron
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1016/j.cellsig.2009.05.002
dc.description.sourcetitleCellular Signalling
dc.description.volume21
dc.description.issue9
dc.description.page1436-1443
dc.description.codenCESIE
dc.identifier.isiut000267787300010
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

19
checked on Dec 2, 2019

WEB OF SCIENCETM
Citations

18
checked on Nov 25, 2019

Page view(s)

61
checked on Nov 30, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.