Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.surfcoat.2005.02.001
Title: Amino-functionalized plasma polymer films for DNA immobilization and hybridization
Authors: Zhang, Z. 
Knoll, W.
Förch, R.
Keywords: Allylamine
DNA immobilization
Hybridization
Plasma polymer
Issue Date: 1-Oct-2005
Citation: Zhang, Z., Knoll, W., Förch, R. (2005-10-01). Amino-functionalized plasma polymer films for DNA immobilization and hybridization. Surface and Coatings Technology 200 (1-4 SPEC. ISS.) : 993-995. ScholarBank@NUS Repository. https://doi.org/10.1016/j.surfcoat.2005.02.001
Abstract: DNA attachment on amine functionalized surfaces has been found to depend on the macromolecular architecture of the plasma films, on the amine group density as well as on the immobilization conditions. Surface Plasmon Resonance spectroscopy (SPR) and Surface Plasmon Enhanced Fluorescence Spectroscopy (SPFS) were used to monitor (a) the immobilization of probe oligomers (25 mers) to plasma polymerised allylamine films (ppA) and (b) their subsequent hybridization reactions with fluorophore-labeled 15 mer target oligonucleotides from solution. To avoid unspecific adsorption of target DNA, any unreacted amino groups were neutralized with succinic anhydride according to standard hybridization procedures. SPF spectroscopic investigations allowed for a precise differentiation between hybridization reactions with mismatch 2 (MM2), mismatch 1 (MM1) and fully complementary (MM0) oligonucleotide target sequences. Complementary target DNA hybridization was shown to have a significantly higher affinity constant (KA) than MM1 and MM2. Low energy plasma deposited allylamine films appear to act as a 3D matrix showing a high capacity towards DNA immobilization and hybridization reactions. © 2005 Elsevier B.V. All rights reserved.
Source Title: Surface and Coatings Technology
URI: http://scholarbank.nus.edu.sg/handle/10635/106961
ISSN: 02578972
DOI: 10.1016/j.surfcoat.2005.02.001
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

32
checked on Oct 23, 2019

WEB OF SCIENCETM
Citations

30
checked on Oct 15, 2019

Page view(s)

31
checked on Oct 11, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.