Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/106768
Title: Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus
Authors: Phang, S.-M. 
Teo, C.-Y.
Lo, E.
Thi Wong, V.W. 
Keywords: DNA sequencing
genomic library
homologies
recombinant
Issue Date: 22-Sep-1995
Citation: Phang, S.-M.,Teo, C.-Y.,Lo, E.,Thi Wong, V.W. (1995-09-22). Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus. Gene 163 (1) : 65-68. ScholarBank@NUS Repository.
Abstract: A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme. © 1995.
Source Title: Gene
URI: http://scholarbank.nus.edu.sg/handle/10635/106768
ISSN: 03781119
Appears in Collections:Staff Publications

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