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https://scholarbank.nus.edu.sg/handle/10635/106768
| Title: | Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus | Authors: | Phang, S.-M. Teo, C.-Y. Lo, E. Thi Wong, V.W. |
Keywords: | DNA sequencing genomic library homologies recombinant |
Issue Date: | 22-Sep-1995 | Citation: | Phang, S.-M.,Teo, C.-Y.,Lo, E.,Thi Wong, V.W. (1995-09-22). Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus. Gene 163 (1) : 65-68. ScholarBank@NUS Repository. | Abstract: | A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme. © 1995. | Source Title: | Gene | URI: | http://scholarbank.nus.edu.sg/handle/10635/106768 | ISSN: | 03781119 |
| Appears in Collections: | Staff Publications |
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