Please use this identifier to cite or link to this item:
|Title:||Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus||Authors:||Phang, S.-M.
Thi Wong, V.W.
|Issue Date:||22-Sep-1995||Citation:||Phang, S.-M.,Teo, C.-Y.,Lo, E.,Thi Wong, V.W. (1995-09-22). Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus. Gene 163 (1) : 65-68. ScholarBank@NUS Repository.||Abstract:||A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme. © 1995.||Source Title:||Gene||URI:||http://scholarbank.nus.edu.sg/handle/10635/106768||ISSN:||03781119|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 22, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.