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Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/106768
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dc.titleCloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus
dc.contributor.authorPhang, S.-M.
dc.contributor.authorTeo, C.-Y.
dc.contributor.authorLo, E.
dc.contributor.authorThi Wong, V.W.
dc.date.accessioned2014-10-29T07:53:25Z
dc.date.available2014-10-29T07:53:25Z
dc.date.issued1995-09-22
dc.identifier.citationPhang, S.-M.,Teo, C.-Y.,Lo, E.,Thi Wong, V.W. (1995-09-22). Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus. Gene 163 (1) : 65-68. ScholarBank@NUS Repository.
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/106768
dc.description.abstractA fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme. © 1995.
dc.sourceScopus
dc.subjectDNA sequencing
dc.subjectgenomic library
dc.subjecthomologies
dc.subjectrecombinant
dc.typeArticle
dc.contributor.departmentZOOLOGY
dc.description.sourcetitleGene
dc.description.volume163
dc.description.issue1
dc.description.page65-68
dc.description.codenGENED
dc.identifier.isiutNOT_IN_WOS
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