Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/102239
Title: Sperm-mediated gene transfer studies on zebrafish in Singapore
Authors: Khoo, H.-W. 
Keywords: Singapore
Sperm-mediated gene transfer
Zebrafish
Issue Date: 2000
Citation: Khoo, H.-W. (2000). Sperm-mediated gene transfer studies on zebrafish in Singapore. Molecular Reproduction and Development 56 (2 SUPPL.) : 278-280. ScholarBank@NUS Repository.
Abstract: This paper describes the work conducted in the Department of Biological Sciences, National University of Singapore, on sperm-mediated gene transfer in zebrafish. This study began by carrying out direct interaction experiments of pUSVCAT reporter DNA with spermatozoa. Other constructs, including pXGH5, pMTL, pRSVL and pGEM-luc, were subsequently used. The different constructs were taken up by sperm cells with comparable efficiencies. In general, no reporter gene expression, Mendelian inheritance, or evidence of genomic integration of the foreign sequences were obtained. However, transmission of the reporter DNA through generations was observed. DNA uptake by sperm cells was shown using FISH and was enhanced by electroporation. The potential use of more recent approaches, such as REMI and ICSI are explored. Future research directions are discussed. (C) 2000 Wiley-Liss, Inc.
Source Title: Molecular Reproduction and Development
URI: http://scholarbank.nus.edu.sg/handle/10635/102239
ISSN: 1040452X
Appears in Collections:Staff Publications

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