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Title: Yeast expression and characterization of SARS-CoV N protein
Authors: Cao, S.
Wang, H. 
Luhur, A.
Wong, S.-M. 
Keywords: N protein
Pichia pastoris
Yeast expression
Issue Date: Dec-2005
Citation: Cao, S., Wang, H., Luhur, A., Wong, S.-M. (2005-12). Yeast expression and characterization of SARS-CoV N protein. Journal of Virological Methods 130 (1-2) : 83-88. ScholarBank@NUS Repository.
Abstract: The severe acute respiratory syndrome human coronavirus (SARS-CoV) nucleocapsid protein (N protein) is its most antigenic structural protein. The N protein gene has been cloned into a yeast expression vector pPIC9, transformed into Pichia pastoris strain GS115 and induced for expression by methanol. SDS-PAGE and Western blot showed that the N protein was expressed at a level of 3 mg/ml of culture medium. Characterization by mass spectrometry, circular dichroism and fluorescence luminescence assays showed that the expressed N protein displayed a β-sheet secondary structure in solution and it is stable in the pH range between 5.0 and 8.0. The P. pastoris-expressed N protein is believed to more closely resemble native SARS N protein than the bacterially expressed N protein. © 2005 Elsevier B.V. All rights reserved.
Source Title: Journal of Virological Methods
ISSN: 01660934
DOI: 10.1016/j.jviromet.2005.06.010
Appears in Collections:Staff Publications

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