Yeast expression and characterization of SARS-CoV N protein

Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jviromet.2005.06.010

Title:	Yeast expression and characterization of SARS-CoV N protein
Authors:	Cao, S. Wang, H. Luhur, A. Wong, S.-M.
Keywords:	N protein Pichia pastoris SARS-CoV Yeast expression
Issue Date:	Dec-2005
Citation:	Cao, S., Wang, H., Luhur, A., Wong, S.-M. (2005-12). Yeast expression and characterization of SARS-CoV N protein. Journal of Virological Methods 130 (1-2) : 83-88. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jviromet.2005.06.010
Abstract:	The severe acute respiratory syndrome human coronavirus (SARS-CoV) nucleocapsid protein (N protein) is its most antigenic structural protein. The N protein gene has been cloned into a yeast expression vector pPIC9, transformed into Pichia pastoris strain GS115 and induced for expression by methanol. SDS-PAGE and Western blot showed that the N protein was expressed at a level of 3 mg/ml of culture medium. Characterization by mass spectrometry, circular dichroism and fluorescence luminescence assays showed that the expressed N protein displayed a β-sheet secondary structure in solution and it is stable in the pH range between 5.0 and 8.0. The P. pastoris-expressed N protein is believed to more closely resemble native SARS N protein than the bacterially expressed N protein. © 2005 Elsevier B.V. All rights reserved.
Source Title:	Journal of Virological Methods
URI:	http://scholarbank.nus.edu.sg/handle/10635/102174
ISSN:	01660934
DOI:	10.1016/j.jviromet.2005.06.010
Appears in Collections:	Staff Publications

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