Please use this identifier to cite or link to this item:
|Title:||The SAM domain of the RhoGAP DLC1 binds EF1A1 to regulate cell migration||Authors:||Zhong, D.
|Issue Date:||1-Feb-2009||Citation:||Zhong, D., Zhang, J., Yang, S., Soh, U.J.K., Buschdorf, J.P., Zhou, Y.T., Yang, D., Low, B.C. (2009-02-01). The SAM domain of the RhoGAP DLC1 binds EF1A1 to regulate cell migration. Journal of Cell Science 122 (3) : 414-424. ScholarBank@NUS Repository. https://doi.org/10.1242/jcs.027482||Abstract:||Deleted in liver cancer 1 (DLC1) is a multi-modular Rho-GTPase-activating protein (RhoGAP) and a tumor suppressor. Besides its RhoGAP domain, functions of other domains in DLC1 remain largely unknown. By protein precipitation and mass spectrometry, we identified eukaryotic elongation factor 1A1 (EF1A1) as a novel partner for the sterile alpha motif (SAM) domain of DLC1 but not the SAM domain of DLC2. The solution structure of DLC1 SAM revealed a new monomeric fold with four parallel helices, similar to that of DLC2 SAM but distinct from other SAM domains. Mutating F38, L39 and F40 within a hydrophobic patch retained its overall structure but abolished its interaction with EF1A1 with F38 and L39 forming an indispensable interacting motif. DLC1 SAM did not localize to and was not required for DLC1 to suppress the turnover of focal adhesions. Instead, DLC1 SAM facilitated EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation. Compared with wild-type DLC1, the non-interactive DLC1 mutant is less potent in suppressing cell migration, whereas overexpression of the DLC1 SAM domain alone, but not the non-interactive mutant SAM or DLC2 SAM, greatly enhanced cell migration. This finding reveals a novel contribution of the SAM-EF1A1 interaction as a potentially important GAP-independent modulation of cell migration by DLC1.||Source Title:||Journal of Cell Science||URI:||http://scholarbank.nus.edu.sg/handle/10635/102005||ISSN:||00219533||DOI:||10.1242/jcs.027482|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 11, 2022
WEB OF SCIENCETM
checked on Aug 11, 2022
checked on Aug 4, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.