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|Title:||Ribosome Biogenesis Factor Bms1-like Is Essential for Liver Development in Zebrafish||Authors:||Wang, Y.
Digestive organ development
|Issue Date:||20-Sep-2012||Citation:||Wang, Y., Luo, Y., Hong, Y., Peng, J., Lo, L. (2012-09-20). Ribosome Biogenesis Factor Bms1-like Is Essential for Liver Development in Zebrafish. Journal of Genetics and Genomics 39 (9) : 451-462. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jgg.2012.07.007||Abstract:||Ribosome biogenesis in the nucleolus requires numerous nucleolar proteins and small non-coding RNAs. Among them is ribosome biogenesis factor Bms1, which is highly conserved from yeast to human. In yeast, Bms1 initiates ribosome biogenesis through recruiting Rcl1 to pre-ribosomes. However, little is known about the biological function of Bms1 in vertebrates. Here we report that Bms1 plays an essential role in zebrafish liver development. We identified a zebrafish bms1lsq163 mutant which carries a T to A mutation in the gene bms1-like (bms1l). This mutation results in L152 to Q152 substitution in a GTPase motif in Bms1l. Surprisingly, bms1lsq163 mutation confers hypoplasia specifically in the liver, exocrine pancreas and intestine after 3 days post-fertilization (dpf). Consistent with the bms1lsq163 mutant phenotypes, whole-mount in situ hybridization (WISH) on wild type embryos showed that bms1l transcripts are abundant in the entire digestive tract and its accessory organs. Immunostaining for phospho-Histone 3 (P-H3) and TUNEL assay revealed that impairment of hepatoblast proliferation rather than cell apoptosis is one of the consequences of bms1lsq163 giving rise to an under-developed liver. Therefore, our findings demonstrate that Bms1l is necessary for zebrafish liver development. © 2012.||Source Title:||Journal of Genetics and Genomics||URI:||http://scholarbank.nus.edu.sg/handle/10635/101600||ISSN:||16738527||DOI:||10.1016/j.jgg.2012.07.007|
|Appears in Collections:||Staff Publications|
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