Please use this identifier to cite or link to this item:
Title: Ribosome Biogenesis Factor Bms1-like Is Essential for Liver Development in Zebrafish
Authors: Wang, Y.
Luo, Y.
Hong, Y. 
Peng, J.
Lo, L. 
Keywords: Bms1-like
Digestive organ development
Liver development
Ribosome biogenesis
Issue Date: 20-Sep-2012
Citation: Wang, Y., Luo, Y., Hong, Y., Peng, J., Lo, L. (2012-09-20). Ribosome Biogenesis Factor Bms1-like Is Essential for Liver Development in Zebrafish. Journal of Genetics and Genomics 39 (9) : 451-462. ScholarBank@NUS Repository.
Abstract: Ribosome biogenesis in the nucleolus requires numerous nucleolar proteins and small non-coding RNAs. Among them is ribosome biogenesis factor Bms1, which is highly conserved from yeast to human. In yeast, Bms1 initiates ribosome biogenesis through recruiting Rcl1 to pre-ribosomes. However, little is known about the biological function of Bms1 in vertebrates. Here we report that Bms1 plays an essential role in zebrafish liver development. We identified a zebrafish bms1lsq163 mutant which carries a T to A mutation in the gene bms1-like (bms1l). This mutation results in L152 to Q152 substitution in a GTPase motif in Bms1l. Surprisingly, bms1lsq163 mutation confers hypoplasia specifically in the liver, exocrine pancreas and intestine after 3 days post-fertilization (dpf). Consistent with the bms1lsq163 mutant phenotypes, whole-mount in situ hybridization (WISH) on wild type embryos showed that bms1l transcripts are abundant in the entire digestive tract and its accessory organs. Immunostaining for phospho-Histone 3 (P-H3) and TUNEL assay revealed that impairment of hepatoblast proliferation rather than cell apoptosis is one of the consequences of bms1lsq163 giving rise to an under-developed liver. Therefore, our findings demonstrate that Bms1l is necessary for zebrafish liver development. © 2012.
Source Title: Journal of Genetics and Genomics
ISSN: 16738527
DOI: 10.1016/j.jgg.2012.07.007
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on May 4, 2021


checked on Apr 26, 2021

Page view(s)

checked on May 2, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.