Please use this identifier to cite or link to this item:
Title: Rapid identification and isolation of zebrafish cDNA clones
Authors: Gong, Z. 
Yan, T.
Liao, J.
Lee, S.E.
He, J. 
Hew, C.L.
Keywords: Embryo
Issue Date: 12-Nov-1997
Citation: Gong, Z., Yan, T., Liao, J., Lee, S.E., He, J., Hew, C.L. (1997-11-12). Rapid identification and isolation of zebrafish cDNA clones. Gene 201 (1-2) : 87-98. ScholarBank@NUS Repository.
Abstract: A fast and economical approach, referred to as cDNA clone tagging, was adapted to identify and isolate zebrafish cDNA clones. The basic approach was to partially sequence the coding region of size selected cDNA clones and the partial sequences were then used as tags for identifying the clones through homology search. To benefit maximally from the tagging approach, two cDNA libraries, derived from embryonic and adult fish poly(A)+ RNAs, respectively, were constructed by unidirectional cloning: conceptually, they have the potential to represent all expressed zebrafish genes. A total of 1084 clones were sequenced from the two libraries, and 511 clones were identified, based on sequence homology. These identified clones were derived from at least 261 genes, encoding 48 translational machinery proteins, 47 cytosolic proteins, 43 cytoskeletal proteins, 41 nuclear proteins, 32 membrane proteins, 22 secreted proteins, 20 mitochondrial proteins and 8 proteins with an unknown location. Of the 261 distinct cDNA clones identified, 254 were isolated for the first time in the zebrafish. These tagged cDNA clones, identified and unidentified, provide rich resources for developmental analysis as well as mapping of zebrafish genome. The long-term objective of this study is to establish a tagged zebrafish gene library that can be accessed both by hybridization screening against the plasmid DNAs and by electronic screening using the sequence information.
Source Title: Gene
ISSN: 03781119
DOI: 10.1016/S0378-1119(97)00431-9
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 14, 2021


checked on Oct 14, 2021

Page view(s)

checked on Oct 14, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.