Please use this identifier to cite or link to this item:
Title: Protection of goldfish against Ichthyophthirius multifiliis by immunization with a recombinant vaccine
Authors: Jiangyan, H. 
Zhan, Y.
Guoliang, X.
Gong, Z. 
Lam, T.J. 
Sin, Y.M. 
Keywords: Ichthyophthirius multifiliis
Immune protection
Recombinant vaccine
Issue Date: 1-Dec-1997
Citation: Jiangyan, H., Zhan, Y., Guoliang, X., Gong, Z., Lam, T.J., Sin, Y.M. (1997-12-01). Protection of goldfish against Ichthyophthirius multifiliis by immunization with a recombinant vaccine. Aquaculture 158 (1-2) : 1-10. ScholarBank@NUS Repository.
Abstract: A 316 bp gene fragment containing a potential antigenic epitope of the 48 kDa immobilization antigen of Ichthyophthirius multifiliis (i-AgI) was assembled from six synthetic oligonucleotides and cloned into a bacterial expression vector pGEX2T. The gene construct was introduced into Escherichia coli and the glutathione S-transferase-iAgI (GST-iAgI) fusion protein was successfully expressed. Antisera against GST-iAgI fusion protein from catfish showed a positive reaction with a tomites protein of about 48 kDa, suggesting that the recombinant protein contains an antigenic epitope of i-AgI. Naive goldfish that were immunized with purified GST-iAgI fusion protein were challenged with a lethal dose of infectious tomites of I. multifiliis. The results showed that the average survival rate of the immunized fish was 95% as compared to 55% for the control fish. All these findings suggest that the recombinant GST-iAgI fusion protein can be used as a potential vaccine against the infection of I. multifiliis.
Source Title: Aquaculture
ISSN: 00448486
DOI: 10.1016/S0044-8486(97)00074-4
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 8, 2019


checked on Oct 8, 2019

Page view(s)

checked on Oct 12, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.