Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0044-8486(97)00074-4
Title: Protection of goldfish against Ichthyophthirius multifiliis by immunization with a recombinant vaccine
Authors: Jiangyan, H. 
Zhan, Y.
Guoliang, X.
Gong, Z. 
Lam, T.J. 
Sin, Y.M. 
Keywords: Ichthyophthirius multifiliis
Immune protection
Recombinant vaccine
Issue Date: 1-Dec-1997
Citation: Jiangyan, H., Zhan, Y., Guoliang, X., Gong, Z., Lam, T.J., Sin, Y.M. (1997-12-01). Protection of goldfish against Ichthyophthirius multifiliis by immunization with a recombinant vaccine. Aquaculture 158 (1-2) : 1-10. ScholarBank@NUS Repository. https://doi.org/10.1016/S0044-8486(97)00074-4
Abstract: A 316 bp gene fragment containing a potential antigenic epitope of the 48 kDa immobilization antigen of Ichthyophthirius multifiliis (i-AgI) was assembled from six synthetic oligonucleotides and cloned into a bacterial expression vector pGEX2T. The gene construct was introduced into Escherichia coli and the glutathione S-transferase-iAgI (GST-iAgI) fusion protein was successfully expressed. Antisera against GST-iAgI fusion protein from catfish showed a positive reaction with a tomites protein of about 48 kDa, suggesting that the recombinant protein contains an antigenic epitope of i-AgI. Naive goldfish that were immunized with purified GST-iAgI fusion protein were challenged with a lethal dose of infectious tomites of I. multifiliis. The results showed that the average survival rate of the immunized fish was 95% as compared to 55% for the control fish. All these findings suggest that the recombinant GST-iAgI fusion protein can be used as a potential vaccine against the infection of I. multifiliis.
Source Title: Aquaculture
URI: http://scholarbank.nus.edu.sg/handle/10635/101471
ISSN: 00448486
DOI: 10.1016/S0044-8486(97)00074-4
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