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https://doi.org/10.1111/j.1755-0998.2008.02221.x
Title: | Improved COI barcoding primers for Southeast Asian perching birds (Aves: Passeriformes) | Authors: | Lohman, D.J. Prawiradilaga, D.M. Meier, R. |
Keywords: | Aves cox 1 Cryptic species DNA barcoding mtDNA Songbirds |
Issue Date: | Jan-2009 | Citation: | Lohman, D.J., Prawiradilaga, D.M., Meier, R. (2009-01). Improved COI barcoding primers for Southeast Asian perching birds (Aves: Passeriformes). Molecular Ecology Resources 9 (1) : 37-40. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1755-0998.2008.02221.x | Abstract: | The All Birds Barcoding Initiative aims to assemble a DNA barcode database for all bird species, but the 648-bp 'barcoding' region of cytochrome c oxidase subunit I (COI) can be difficult to amplify in Southeast Asian perching birds (Aves: Passeriformes). Using COI sequences from complete mitochondrial genomes, we designed a primer pair that more reliably amplifies and sequences the COI barcoding region of Southeast Asian passerine birds. The 655-bp region amplified with these primers overlaps the COI region amplified with other barcoding primer pairs, enabling direct comparison of sequences with previously published DNA barcodes. © 2008 The Authors. | Source Title: | Molecular Ecology Resources | URI: | http://scholarbank.nus.edu.sg/handle/10635/100894 | ISSN: | 1755098X | DOI: | 10.1111/j.1755-0998.2008.02221.x |
Appears in Collections: | Staff Publications |
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