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Title: Improved COI barcoding primers for Southeast Asian perching birds (Aves: Passeriformes)
Authors: Lohman, D.J. 
Prawiradilaga, D.M.
Meier, R. 
Keywords: Aves
cox 1
Cryptic species
DNA barcoding
Issue Date: Jan-2009
Citation: Lohman, D.J., Prawiradilaga, D.M., Meier, R. (2009-01). Improved COI barcoding primers for Southeast Asian perching birds (Aves: Passeriformes). Molecular Ecology Resources 9 (1) : 37-40. ScholarBank@NUS Repository.
Abstract: The All Birds Barcoding Initiative aims to assemble a DNA barcode database for all bird species, but the 648-bp 'barcoding' region of cytochrome c oxidase subunit I (COI) can be difficult to amplify in Southeast Asian perching birds (Aves: Passeriformes). Using COI sequences from complete mitochondrial genomes, we designed a primer pair that more reliably amplifies and sequences the COI barcoding region of Southeast Asian passerine birds. The 655-bp region amplified with these primers overlaps the COI region amplified with other barcoding primer pairs, enabling direct comparison of sequences with previously published DNA barcodes. © 2008 The Authors.
Source Title: Molecular Ecology Resources
ISSN: 1755098X
DOI: 10.1111/j.1755-0998.2008.02221.x
Appears in Collections:Staff Publications

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