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Title: Generation of medaka fish haploid embryonic stem cells
Authors: Yi, M. 
Hong, N.
Hong, Y. 
Issue Date: 16-Oct-2009
Citation: Yi, M., Hong, N., Hong, Y. (2009-10-16). Generation of medaka fish haploid embryonic stem cells. Science 326 (5951) : 430-433. ScholarBank@NUS Repository.
Abstract: Haploid embryonic stem (ES) cells combine haploidy and pluripotency, enabling direct genetic analyses of recessive phenotypes in vertebrate cells. Haploid cells have been elusive for culture, due to their inferior growth and genomic instability. Here, we generated gynogenetic medaka embryos and obtained three haploid ES cell lines that retained pluripotency and competitive growth. Upon nuclear transfer into unfertilized oocytes, the haploid ES cells, even after genetic engineering, generated viable offspring capable of germline transmission. Hence, haploid medaka ES cells stably maintain normal growth, pluripotency, and genomic integrity. Mosaic oocytes created by combining a mitotic nucleus and a meiotic nucleus can generate fertile fish offspring. Haploid ES cells may offer a yeast-like system for analyzing recessive phenotypes in numerous cell lineages of vertebrates in vitro.
Source Title: Science
ISSN: 00368075
DOI: 10.1126/science.1175151
Appears in Collections:Staff Publications

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