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https://doi.org/10.1023/A:1026764514737
| Title: | Functional expression of full length Limulus Factor C in stably transformed Sf9 cells | Authors: | Wang, J. Bow Ho Ding, J.L. |
Keywords: | Endotoxin-binding Expression Recombinant Factor C Secretion Spodoptera frugiperda Sf9 |
Issue Date: | 2001 | Citation: | Wang, J., Bow Ho, Ding, J.L. (2001). Functional expression of full length Limulus Factor C in stably transformed Sf9 cells. Biotechnology Letters 23 (1) : 71-76. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1026764514737 | Abstract: | Limulus Factor C is a potent antagonist of endotoxin from Gram-negative bacteria. A fusion construct containing full length Factor C has been cloned into Spodoptera frugiperda Sf9 cell. Stable Sf9 cell transfectants were obtained using Zeocin selection for 2 weeks. The recombinant Factor C (rFC) was secreted into the culture medium at 9 mg 1-1. Both the crude and partially purified rFC were able to detect lipid A at 10 pg ml-1 in an ELISA-based lipid A binding assay. | Source Title: | Biotechnology Letters | URI: | http://scholarbank.nus.edu.sg/handle/10635/100723 | ISSN: | 01415492 | DOI: | 10.1023/A:1026764514737 |
| Appears in Collections: | Staff Publications |
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