Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1026764514737
Title: Functional expression of full length Limulus Factor C in stably transformed Sf9 cells
Authors: Wang, J. 
Ho, B.
Ding, J.L. 
Keywords: Endotoxin-binding
Expression
Recombinant Factor C
Secretion
Spodoptera frugiperda Sf9
Issue Date: 2001
Citation: Wang, J., Ho, B., Ding, J.L. (2001). Functional expression of full length Limulus Factor C in stably transformed Sf9 cells. Biotechnology Letters 23 (1) : 71-76. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1026764514737
Abstract: Limulus Factor C is a potent antagonist of endotoxin from Gram-negative bacteria. A fusion construct containing full length Factor C has been cloned into Spodoptera frugiperda Sf9 cell. Stable Sf9 cell transfectants were obtained using Zeocin selection for 2 weeks. The recombinant Factor C (rFC) was secreted into the culture medium at 9 mg 1-1. Both the crude and partially purified rFC were able to detect lipid A at 10 pg ml-1 in an ELISA-based lipid A binding assay.
Source Title: Biotechnology Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/100723
ISSN: 01415492
DOI: 10.1023/A:1026764514737
Appears in Collections:Staff Publications

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