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Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1026764514737
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dc.titleFunctional expression of full length Limulus Factor C in stably transformed Sf9 cells
dc.contributor.authorWang, J.
dc.contributor.authorHo, B.
dc.contributor.authorDing, J.L.
dc.date.accessioned2014-10-27T08:29:00Z
dc.date.available2014-10-27T08:29:00Z
dc.date.issued2001
dc.identifier.citationWang, J., Ho, B., Ding, J.L. (2001). Functional expression of full length Limulus Factor C in stably transformed Sf9 cells. Biotechnology Letters 23 (1) : 71-76. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1026764514737
dc.identifier.issn01415492
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100723
dc.description.abstractLimulus Factor C is a potent antagonist of endotoxin from Gram-negative bacteria. A fusion construct containing full length Factor C has been cloned into Spodoptera frugiperda Sf9 cell. Stable Sf9 cell transfectants were obtained using Zeocin selection for 2 weeks. The recombinant Factor C (rFC) was secreted into the culture medium at 9 mg 1-1. Both the crude and partially purified rFC were able to detect lipid A at 10 pg ml-1 in an ELISA-based lipid A binding assay.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1023/A:1026764514737
dc.sourceScopus
dc.subjectEndotoxin-binding
dc.subjectExpression
dc.subjectRecombinant Factor C
dc.subjectSecretion
dc.subjectSpodoptera frugiperda Sf9
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1023/A:1026764514737
dc.description.sourcetitleBiotechnology Letters
dc.description.volume23
dc.description.issue1
dc.description.page71-76
dc.description.codenBILED
dc.identifier.isiut000165813800014
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