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|Title:||Expression, purification and crystallization of a novel nonstructural protein VP9 from white spot syndrome virus||Authors:||Liu, Y.
|Issue Date:||Aug-2006||Citation:||Liu, Y., Sivaraman, J., Hew, C.L. (2006-08). Expression, purification and crystallization of a novel nonstructural protein VP9 from white spot syndrome virus. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 62 (8) : 802-804. ScholarBank@NUS Repository. https://doi.org/10.1107/S1744309106027436||Abstract:||The nonstructural protein VP9 from white spot syndrome virus (WSSV) has been identified and expressed in Escherichia coli. To facilitate purification, a cleavable His6 tag was introduced at the N-terminus. The native protein was purified and crystallized by vapour diffusion against mother liquor containing 2 M sodium acetate, 100 mM MES pH 6.3, 25 mM cadmium sulfate and 3% glycerol. Crystals were obtained within 7 d and diffracted to 2.2 Å; they belonged to space group P212121, with unit-cell parameters a = 74.13, b = 78.21, c = 78.98 Å and four molecules in the asymmetric unit. The selenomethionine-labelled protein produced isomorphous crystals that diffracted to approximately 3.3 Å. © 2006 International Union of Crystallography All rights reserved.||Source Title:||Acta Crystallographica Section F: Structural Biology and Crystallization Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/100651||ISSN:||17443091||DOI:||10.1107/S1744309106027436|
|Appears in Collections:||Staff Publications|
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