Please use this identifier to cite or link to this item: https://doi.org/10.1107/S1744309106027436
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dc.titleExpression, purification and crystallization of a novel nonstructural protein VP9 from white spot syndrome virus
dc.contributor.authorLiu, Y.
dc.contributor.authorSivaraman, J.
dc.contributor.authorHew, C.L.
dc.date.accessioned2014-10-27T08:28:12Z
dc.date.available2014-10-27T08:28:12Z
dc.date.issued2006-08
dc.identifier.citationLiu, Y., Sivaraman, J., Hew, C.L. (2006-08). Expression, purification and crystallization of a novel nonstructural protein VP9 from white spot syndrome virus. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 62 (8) : 802-804. ScholarBank@NUS Repository. https://doi.org/10.1107/S1744309106027436
dc.identifier.issn17443091
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100651
dc.description.abstractThe nonstructural protein VP9 from white spot syndrome virus (WSSV) has been identified and expressed in Escherichia coli. To facilitate purification, a cleavable His6 tag was introduced at the N-terminus. The native protein was purified and crystallized by vapour diffusion against mother liquor containing 2 M sodium acetate, 100 mM MES pH 6.3, 25 mM cadmium sulfate and 3% glycerol. Crystals were obtained within 7 d and diffracted to 2.2 Å; they belonged to space group P212121, with unit-cell parameters a = 74.13, b = 78.21, c = 78.98 Å and four molecules in the asymmetric unit. The selenomethionine-labelled protein produced isomorphous crystals that diffracted to approximately 3.3 Å. © 2006 International Union of Crystallography All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1107/S1744309106027436
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1107/S1744309106027436
dc.description.sourcetitleActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.description.volume62
dc.description.issue8
dc.description.page802-804
dc.identifier.isiut000239357900024
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