Please use this identifier to cite or link to this item: https://doi.org/10.1002/jez.b.21301
Title: Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp
Authors: Peng, J.-X.
Xie, J.-L.
Zhou, L.
Hong, Y.-H. 
Gui, J.-F.
Issue Date: Dec-2009
Citation: Peng, J.-X., Xie, J.-L., Zhou, L., Hong, Y.-H., Gui, J.-F. (2009-12). Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 312 (8) : 855-871. ScholarBank@NUS Repository. https://doi.org/10.1002/jez.b.21301
Abstract: To investigate germline development and germ cell specification, we identified a Dazl homolog (CagDazl) from gynogenetic gibel carp (Carassius auratus gibelio). Its cDNA sequence and BAC clone sequence analyses revealed the genomic organization conservation and conserved synteny of the Dazl family members and their neighborhood genes among vertebrates, especially in fish. Moreover, a polyclonal antibody specific to CagDazl was produced and used to examine its expression and distribution throughout germline development at protein level. Firstly, ovary-specific expression pattern of CagDazl was confirmed in adult tissues by RT-PCR and Western blot. In addition, in situ hybridization and immunofluorescence localization demonstrated its specific expression in germ cells, and both its transcript and protein were localized to germ plasm. Then, co-localization of CagDazl and mitochondrial cloud was found, confirming that CagDazl transcript and its protein are germ plasm component and move via METRO pathway during oogenesis. Furthermore, the CagDazl is abundant and continuous throughout germline development and germ cell specification including primordial germ cell (PGC) formation, oogonium differentiation, oocyte development, and embryogenesis, and the dynamic distribution occurs at different development stages. The data suggest that maternal CagDazl might play an important role in gibel carp PGC formation. Therefore, CagDazl is a useful and specific marker for tracing germ plasm and germ cell development in the gynogenetic gibel carp. In addition, in comparison with previous studies in sexual reproduction species, the continuous and dynamic distribution of CagDazl protein in the germ plasm throughout the life cycle seems to have significant implication in sex evolution of vertebrates. © 2009 Wiley-Liss, Inc.
Source Title: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
URI: http://scholarbank.nus.edu.sg/handle/10635/100622
ISSN: 15525007
DOI: 10.1002/jez.b.21301
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