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Title: Enzyme-stabilizing activity of seed trypsin inhibitors during desiccation
Authors: Lam, J.-M.
Pwee, K.-H. 
Sun, W.Q. 
Chua, Y.-L.
Wang, X.-J.
Keywords: Adenanthera pavonina
Enzyme preservation
Glucose-6-phosphate dehydrogenase
Kunitz trypsin inhibitor
Protein stabilization
Seed desiccation
Issue Date: 29-Mar-1999
Citation: Lam, J.-M., Pwee, K.-H., Sun, W.Q., Chua, Y.-L., Wang, X.-J. (1999-03-29). Enzyme-stabilizing activity of seed trypsin inhibitors during desiccation. Plant Science 142 (2) : 209-218. ScholarBank@NUS Repository.
Abstract: Seeds contain protective proteins which are able to preserve glucose-6- phosphate dehydrogenase (EC activity upon co-dehydration of the enzyme with heat-stable seed protein extracts. Adenanthera pavonina L. (saga) seed extracts, in particular, have high levels of enzyme-preserving activity which not only alleviate the detrimental effects of desiccation in the presence of Tris-HCl and NaCl, but also stabilize enzyme activity in solution. Purification of Adenanthera seed extracts by reversed-phase and weak anion-exchange perfusion chromatography, followed by N-terminal protein sequencing have identified Kunitz-type trypsin inhibitors as the major proteins in one set of enzyme-preserving fractions. A quantitative comparison using various concentrations of purified proteins has demonstrated that soybean Kunitz trypsin inhibitor is as effective (on a mass basis) as bovine serum albumin in preserving enzyme activity, and may be an important protein permitting enzyme dehydration in a seed undergoing desiccation.
Source Title: Plant Science
ISSN: 01689452
DOI: 10.1016/S0168-9452(99)00007-2
Appears in Collections:Staff Publications

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