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https://doi.org/10.1016/S0168-9452(99)00007-2
Title: | Enzyme-stabilizing activity of seed trypsin inhibitors during desiccation | Authors: | Lam, J.-M. Pwee, K.-H. Sun, W.Q. Chua, Y.-L. Wang, X.-J. |
Keywords: | Adenanthera pavonina Enzyme preservation Glucose-6-phosphate dehydrogenase Kunitz trypsin inhibitor Protein stabilization Seed desiccation |
Issue Date: | 29-Mar-1999 | Citation: | Lam, J.-M., Pwee, K.-H., Sun, W.Q., Chua, Y.-L., Wang, X.-J. (1999-03-29). Enzyme-stabilizing activity of seed trypsin inhibitors during desiccation. Plant Science 142 (2) : 209-218. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-9452(99)00007-2 | Abstract: | Seeds contain protective proteins which are able to preserve glucose-6- phosphate dehydrogenase (EC 1.1.1.49) activity upon co-dehydration of the enzyme with heat-stable seed protein extracts. Adenanthera pavonina L. (saga) seed extracts, in particular, have high levels of enzyme-preserving activity which not only alleviate the detrimental effects of desiccation in the presence of Tris-HCl and NaCl, but also stabilize enzyme activity in solution. Purification of Adenanthera seed extracts by reversed-phase and weak anion-exchange perfusion chromatography, followed by N-terminal protein sequencing have identified Kunitz-type trypsin inhibitors as the major proteins in one set of enzyme-preserving fractions. A quantitative comparison using various concentrations of purified proteins has demonstrated that soybean Kunitz trypsin inhibitor is as effective (on a mass basis) as bovine serum albumin in preserving enzyme activity, and may be an important protein permitting enzyme dehydration in a seed undergoing desiccation. | Source Title: | Plant Science | URI: | http://scholarbank.nus.edu.sg/handle/10635/100595 | ISSN: | 01689452 | DOI: | 10.1016/S0168-9452(99)00007-2 |
Appears in Collections: | Staff Publications |
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