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Title: Development of transgenic fish for ornamental and bioreactor by strong expression of fluorescent proteins in the skeletal muscle
Authors: Gong, Z. 
Wan, H.
Tay, T.L.
Wang, H. 
Chen, M.
Yan, T.
Keywords: dsRed
Generically modified organism
Green fluorescent protein
Red fluorescent protein
Yellow fluorescent protein
Issue Date: 15-Aug-2003
Citation: Gong, Z., Wan, H., Tay, T.L., Wang, H., Chen, M., Yan, T. (2003-08-15). Development of transgenic fish for ornamental and bioreactor by strong expression of fluorescent proteins in the skeletal muscle. Biochemical and Biophysical Research Communications 308 (1) : 58-63. ScholarBank@NUS Repository.
Abstract: In the present study, new applications of the transgenic technology in developing novel varieties of ornamental fish and bioreactor fish were explored in a model fish, the zebrafish (Danio rerio). Three "living color" fluorescent proteins, green fluorescent protein (GFP), yellow fluorescent protein (YFP), and red fluorescent protein (RFP or dsRed), were expressed under a strong muscle-specific mylz2 promoter in stable lines of transgenic zebrafish. These transgenic zebrafish display vivid fluorescent colors (green, red, yellow, or orange) visible to unaided eyes under both daylight and ultraviolet light in the dark. The level of foreign protein expression is estimated between 3% and 17% of total muscle proteins, equivalent to 4.8-27.2mg/g wet muscle tissue. Thus, the fish muscle may be explored as another useful bioreactor system for production of recombinant proteins. In spite of the high level of foreign protein expression, the expression of endogenous mylz2 mRNAs was not negatively affected. Furthermore, compared to the wild-type fish, these fluorescent transgenic fish have no advantage in survival and reproduction. © 2003 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/S0006-291X(03)01282-8
Appears in Collections:Staff Publications

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