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Title: Customisation of AFLP analysis for cassava varietal identification
Authors: Wong, H.-L.
Yeoh, H.-H. 
Lim, S.-H. 
Keywords: AFLP markers
Cyanogenic potential
Manihot esculenta
Selective nucleotides
Varietal identification
Issue Date: 20-Mar-1999
Citation: Wong, H.-L., Yeoh, H.-H., Lim, S.-H. (1999-03-20). Customisation of AFLP analysis for cassava varietal identification. Phytochemistry 50 (6) : 919-924. ScholarBank@NUS Repository.
Abstract: Amplified fragment length polymorphism (AFLP) markers were used in the characterization of eight cassava varieties. This nonradioactive AFLP system was customized in terms of the choice of restriction enzymes used and the selection of nucleotides added to the 3' end of primers. EcoRI/MseI and HindIII/MseI fragments generally gave monomorphic profiles while ApaI/TaqI fragments produced polymorphic profiles suggesting a genome with high G + C content. It was possible to identify the eight cassava varieties used in this study using CTG as selective bases at the TaqI primer. For cassava, the AFLP system provided a higher number of loci detected per run when compared to RAPD. The reliability accompanying AFLP analysis would thus make it suitable for the characterization of cassava varieties.
Source Title: Phytochemistry
ISSN: 00319422
DOI: 10.1016/S0031-9422(98)00628-1
Appears in Collections:Staff Publications

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