Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0031-9422(98)00628-1
DC FieldValue
dc.titleCustomisation of AFLP analysis for cassava varietal identification
dc.contributor.authorWong, H.-L.
dc.contributor.authorYeoh, H.-H.
dc.contributor.authorLim, S.-H.
dc.date.accessioned2014-10-27T08:25:15Z
dc.date.available2014-10-27T08:25:15Z
dc.date.issued1999-03-20
dc.identifier.citationWong, H.-L., Yeoh, H.-H., Lim, S.-H. (1999-03-20). Customisation of AFLP analysis for cassava varietal identification. Phytochemistry 50 (6) : 919-924. ScholarBank@NUS Repository. https://doi.org/10.1016/S0031-9422(98)00628-1
dc.identifier.issn00319422
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100384
dc.description.abstractAmplified fragment length polymorphism (AFLP) markers were used in the characterization of eight cassava varieties. This nonradioactive AFLP system was customized in terms of the choice of restriction enzymes used and the selection of nucleotides added to the 3' end of primers. EcoRI/MseI and HindIII/MseI fragments generally gave monomorphic profiles while ApaI/TaqI fragments produced polymorphic profiles suggesting a genome with high G + C content. It was possible to identify the eight cassava varieties used in this study using CTG as selective bases at the TaqI primer. For cassava, the AFLP system provided a higher number of loci detected per run when compared to RAPD. The reliability accompanying AFLP analysis would thus make it suitable for the characterization of cassava varieties.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0031-9422(98)00628-1
dc.sourceScopus
dc.subjectAFLP markers
dc.subjectCyanogenic potential
dc.subjectEuphorbiaceae
dc.subjectManihot esculenta
dc.subjectSelective nucleotides
dc.subjectVarietal identification
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0031-9422(98)00628-1
dc.description.sourcetitlePhytochemistry
dc.description.volume50
dc.description.issue6
dc.description.page919-924
dc.description.codenPYTCA
dc.identifier.isiut000080077100002
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