Please use this identifier to cite or link to this item:
Title: C-reactive protein: A predominant LPS-binding acute phase protein responsive to Pseudomonas infection
Authors: Ng, P.M.L. 
Jin, Z. 
Tan, S.S.H.
Ho, B.
Ding, J.L. 
Keywords: C-reactive protein
Horseshoe crab
Pseudomonas aeruginosa
Issue Date: 2004
Citation: Ng, P.M.L., Jin, Z., Tan, S.S.H., Ho, B., Ding, J.L. (2004). C-reactive protein: A predominant LPS-binding acute phase protein responsive to Pseudomonas infection. Journal of Endotoxin Research 10 (3) : 163-174. ScholarBank@NUS Repository.
Abstract: As a structural component of the outer membrane of Gram-negative bacteria, endotoxin, also known as lipopolysaccharide (LPS) exhibits strong immunostimulatory properties, rendering it a pivotal role in the pathogenesis of Gram-negative septicaemia. Our attempt to identify LPS-binding proteins from the hemolymph of the horseshoe crab led to the isolation and identification of C-reactive protein (CRP) as the predominant LPS-recognition protein during Pseudomonas infection. CRP is an evolutionarily ancient member of a superfamily of 'pentraxins'. It is a major protein in acute phase of infection in humans. Our investigation of CRP response to Pseudomonas aeruginosa unveiled a robust innate immune system in the horseshoe crab, which displays rapid suppression of a dosage of 106 CFU of bacteria in the first hour of infection and effected complete clearance of the pathogen by 3 days. Such a high dose would have been lethal to mice. Full-length CRP cDNA was cloned. Analysis of the untranslated regions suggests their crucial role in post-transcriptional regulation of CRP transcript levels. Northern blot analysis demonstrated an acute up-regulation of CRP by about 60-fold in 6-48 h of Pseudomonas infection. Taken together, our results provide new insights into the importance of CRP as a conserved molecule for pathogen recognition. © W.S. Maney & Son Ltd.
Source Title: Journal of Endotoxin Research
ISSN: 09680519
DOI: 10.1179/096805104225004833
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jul 15, 2019


checked on Jul 8, 2019

Page view(s)

checked on Jul 20, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.