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https://doi.org/10.1179/096805104225004833
DC Field | Value | |
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dc.title | C-reactive protein: A predominant LPS-binding acute phase protein responsive to Pseudomonas infection | |
dc.contributor.author | Ng, P.M.L. | |
dc.contributor.author | Jin, Z. | |
dc.contributor.author | Tan, S.S.H. | |
dc.contributor.author | Ho, B. | |
dc.contributor.author | Ding, J.L. | |
dc.date.accessioned | 2014-10-27T08:24:51Z | |
dc.date.available | 2014-10-27T08:24:51Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | Ng, P.M.L., Jin, Z., Tan, S.S.H., Ho, B., Ding, J.L. (2004). C-reactive protein: A predominant LPS-binding acute phase protein responsive to Pseudomonas infection. Journal of Endotoxin Research 10 (3) : 163-174. ScholarBank@NUS Repository. https://doi.org/10.1179/096805104225004833 | |
dc.identifier.issn | 09680519 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/100350 | |
dc.description.abstract | As a structural component of the outer membrane of Gram-negative bacteria, endotoxin, also known as lipopolysaccharide (LPS) exhibits strong immunostimulatory properties, rendering it a pivotal role in the pathogenesis of Gram-negative septicaemia. Our attempt to identify LPS-binding proteins from the hemolymph of the horseshoe crab led to the isolation and identification of C-reactive protein (CRP) as the predominant LPS-recognition protein during Pseudomonas infection. CRP is an evolutionarily ancient member of a superfamily of 'pentraxins'. It is a major protein in acute phase of infection in humans. Our investigation of CRP response to Pseudomonas aeruginosa unveiled a robust innate immune system in the horseshoe crab, which displays rapid suppression of a dosage of 106 CFU of bacteria in the first hour of infection and effected complete clearance of the pathogen by 3 days. Such a high dose would have been lethal to mice. Full-length CRP cDNA was cloned. Analysis of the untranslated regions suggests their crucial role in post-transcriptional regulation of CRP transcript levels. Northern blot analysis demonstrated an acute up-regulation of CRP by about 60-fold in 6-48 h of Pseudomonas infection. Taken together, our results provide new insights into the importance of CRP as a conserved molecule for pathogen recognition. © W.S. Maney & Son Ltd. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1179/096805104225004833 | |
dc.source | Scopus | |
dc.subject | C-reactive protein | |
dc.subject | Endotoxin | |
dc.subject | Horseshoe crab | |
dc.subject | Lipopolysaccharide | |
dc.subject | Pseudomonas aeruginosa | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1179/096805104225004833 | |
dc.description.sourcetitle | Journal of Endotoxin Research | |
dc.description.volume | 10 | |
dc.description.issue | 3 | |
dc.description.page | 163-174 | |
dc.description.coden | JENRE | |
dc.identifier.isiut | 000222280500002 | |
Appears in Collections: | Staff Publications |
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