Discovery of a structural-element specific g-quadruplex "light-up" probe
Zhang, L. Er, J.C. Ghosh, K.K. Chung, W.J. Yoo, J. Xu, W. Zhao, W. Phan, A.T. Chang, Y.-T.
Zhang, L.
Er, J.C.
Ghosh, K.K.
Chung, W.J.
Yoo, J.
Xu, W.
Zhao, W.
Phan, A.T.
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Abstract
The development of a fluorescent probe capable of detecting and distinguishing the wide diversity of G-quadruplex structures is particularly challenging. Herein, we report a novel BODIPY-based fluorescent sensor (GQR) that shows unprecedented selectivity to parallel-stranded G-quadruplexes with exposed ends and four medium grooves. Mechanistic studies suggest that GQR associates with G-quadruplex grooves close to the end of the tetrad core, which may explain the dye's specificity to only a subset of parallel structures. This specific recognition favours the disaggregation of GQR in aqueous solutions thereby recovering the inherent fluorescence of the dye. Due to its unique features, GQR represents a valuable tool for basic biological research and the rapid discovery of novel, specific ligands that target similar structural features of G-quadruplexes.
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Scientific Reports
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Date
2014-01-20
DOI
10.1038/srep03776
Type
Article