El Oakley,Reida Menshawe
Email Address
surrmo@nus.edu.sg
Organizational Units
SURGERY
dept
YONG LOO LIN SCH OF MEDICINE
faculty
37 results
Publication Search Results
Now showing 1 - 10 of 37
Publication Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium(2008) Timmers, L.; Arslan, F.; Hoefer, I.E.; Doevendans, P.A.; Pasterkamp, G.; de, Kleijn D.P.V.; Lim, S.K.; Armstrong, J.S.; Piek, J.J.; El, Oakley R.M.; Choo, A.; Lee, C.N.; SURGERY; BIOCHEMISTRYAlthough paracrine effects of mesenchymal stem cells (MSCs) have been suggested previously, cardioprotection by human MSC secretions has never been demonstrated. Human MSC-conditioned medium (CM) was collected by following a clinically compliant protocol. In a porcine model of ischemia and reperfusion injury, intravenous and intracoronary MSC-CM treatment significantly reduced myocardial nuclear oxidative stress as determined by immunostaining for 8-hydroxy-2′-deoxyguanosine. In addition, expression levels of phospho-SMAD2 and active caspase 3 were diminished following CM treatment, suggesting that TGF-β signaling and apoptosis were reduced. This was associated with a 60% reduction in infarct size and marked improvement of systolic and diastolic cardiac performance as assessed with echocardiography and pressure volume loops. Fractionation studies revealed that only the fraction of the CM containing products > 1000 kDa (100-220 nm) provided cardioprotection in a mouse model of ischemia and reperfusion injury. This indicates that the responsible paracrine factor of human MSCs is likely a large complex rather than a single small molecule. These data identify human MSC-CM as a promising therapeutic option to reduce myocardial infarct size in patients with acute MI and suggest that the use of stem cell secretions could extend the applicability of stem cells for therapeutic purposes. © 2008 Elsevier B.V. All rights reserved.Publication Off-pump coronary artery bypass grafting is a safe and effective treatment modality for Asian patients requiring coronary revascularisation(2005) Ashok, V.; Nishkantha, A.; Leong, K.H.; Luo, H.D.; El-Oakley, R.M.; Tan, C.T.T.; Lee, C.N.; Sim, E.K.; SURGERYPublication Homocysteine is positively associated with cytokine IL-18 plasma levels in coronary artery bypass surgery patients(2005) McLachlan, C.S.; Chua, W.C.-N.; Wong, P.T.H.; Ti, L.K.; Chen, C.; El, Oakley R.M.; PHARMACOLOGY; SURGERY; ANAESTHESIAPublication Physiologically compatible cardiac assist device and method(2003-09-18) EL, OAKLEY REIDA MENSHAWE; LIM, HOU-SEN; SURGERYA physiologically compatible cardiac assist device, also known as a ventricular assist device, has a pump which draws blood through a first catheter inserted into either the right atrium, the left atrium or the left ventricle and infuses the blood via a second catheter into the aorta during diastole. Attached to the second catheter is a balloon which is also inflated during diastole. The synchronized volume infusion and balloon inflation in this counterpulsation mode reduces afterload and is thus more physiologically compatible.Publication Composition derived from biological materials and method of use and preparation(2008-08-05) PEDDAREDDIGARI, VIJAY GOPAL REDDY; LIM, TAI TIAN; EL OAKLEY, REIDA MENSHAWE; MEDICINE; SURGERY; NATIONAL HEART CENTER (SINGAPORE, SG); NATIONAL UNIVERSITY OF SINGAPOREThe invention provides a novel composition and methods of its preparation and uses. The composition comprises as main components serum and a gelling agent and is useful in various medical applications. The composition may be used to coat a medical device, as a biological glue, or as dressings, membranes, scaffolding or hydrogel useful in bioengineering applications. One or more therapeutic products may be added to the composition and the composition is also a vehicle for delivery of therapeutic products.Publication Allograft tissue for use in valve replacement(2006) Da, Costa M.L.; El, Oakley R.M.; Al, Ghofaili F.; SURGERYPublication Addendum to "Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury" [Stem Cell Research 4 (2010) 214-222] (DOI:10.1016/j.scr.2009.12.003)(2010) Lai, R.C.; Chen, T.S.; Lim, S.K.; Lai, R.C.; Arslan, F.; Timmers, L.; Pasterkamp, G.; de, Kleijn D.P.V.; Lee, M.M.; Sze, S.K.; Choo, A.; Salto-Tellez, M.; Lee, C.N.; El, Oakley R.M.; de, Kleijn D.P.V.; SURGERY; BIOLOGICAL SCIENCES; DIVISION OF BIOENGINEERING; PATHOLOGYPublication Preoperative microalbuminuria, haptoglobin phenotype 2-2, and age are independent predictors for acute renal failure following coronary artery bypass graft.(2004-09) Luo, H.D.; Ramirez, S.P.; Costa, M.D.; Tan, C.T.; Oakley, R.E.; Lee, C.N.; Hsu, S.I.; MEDICINE; SURGERY; PAEDIATRICSPublication PHYSIOLOGICALLY COMPATIBLE CARDIAC ASSIST DEVICE AND METHOD(2004-12-08) EL OAKLEY, REIDA MENSHAWE; LIM, HOU-SEN; SURGERYA physiologically compatible cardiac assist device has a pump (20) which draws blood through a first catheter (30) inserted into either the right atrium (2), the left atrium (4) or the left ventricle (8) and infuses the blood via a second catheter (32) into the aorta during disatole. Attached to the second catheter is a balloon (24) that is also inflated during diastole. The synchronized volume infusion and balloon inflation in this counter-pulsation mode reduces after load and is thus more physiologically compatible.Publication PHYSIOLOGICALLY COMPATIBLE CARDIAC ASSIST DEVICE AND METHOD(2003-08-21) EL OAKLEY, REIDA MENSHAWE; LIM, HOU-SEN; SURGERYA physiologically compatible cardiac assist device has a pump (20) which draws blood through a first catheter (30) inserted into either the right atrium (2), the left atrium (4) or the left ventricle (8) and infuses the blood via a second catheter (32) into the aorta during disatole. Attached to the second catheter is a balloon (24) that is also inflated during diastole. The synchronized volume infusion and balloon inflation in this counter-pulsation mode reduces after load and is thus more physiologically compatible.