LIONG KA HANG

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miclkh@nus.edu.sg


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    Ap4A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells
    (Elsevier Inc., 2019) La Shu, S.; Paruchuru, L.B.; Tay, N.Q.; Chua, Y.L.; Yun Foo, A.S.; Yang, C.M.; Liong, K.H.; Liang Koh, E.G.; Lee, A.; Nechushtan, H.; Razin, E.; Kemeny, D.M.; MICROBIOLOGY AND IMMUNOLOGY; LIFE SCIENCES INSTITUTE
    The significance of intracellular Ap4A levels over immune activity of dendritic cells (DCs) has been studied in Nudt2fl/fl/CD11c-cre mice. The transgenic mice have been generated by crossing floxed NUDT2 gene mice with DC marker CD11c recombinase (cre) mice. The DCs derived from these mice have higher levels of Ap4A (?30-fold) compared with those derived from Nudt2+/+ mice. Interestingly, the elevated Ap4A in DCs has led them to possess higher motility and lower directional variability. In addition, the DCs are able to enhance immune protection indicated by the higher cross-presentation of antigen and priming of CD8+ OT-I T cells. Overall, the study denotes prominent impact of Ap4A over the functionality of DCs. The Nudt2fl/fl/CD11c-cre mice could serve as a useful tool to study the influence of Ap4A in the critical immune mechanisms of DCs. Molecular Mechanism of Behavior; Immunology; Immune Response; Model Organism © 2019 The Author(s)
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    Publication
    Transitional premonocytes emerge in the periphery for host defense against bacterial infections
    (AMER ASSOC ADVANCEMENT SCIENCE, 2022-03-01) Teh, Ye Chean; Chooi, Ming Yao; Liu, Dehua; Kwok, Immanuel; Lai, Ghee Chuan; Yong, Liyana Ayub Ow; Ng, Melissa; Li, Jackson LY; Tan, Yingrou; Evrard, Maximilien; Tan, Leonard; Liong, Ka Hang; Leong, Keith; Goh, Chi Ching; Chan, Andrew YJ; Shadan, Nurhidaya Binte; Mantri, Chinmay Kumar; Hwang, You Yi; Cheng, Hui; Cheng, Tao; Yu, Weimiao; Tey, Hong Liang; Larbi, Anis; St John, Ashley; Angeli, Veronique; Ruedl, Christiane; Lee, Bernett; Ginhoux, Florent; Chen, Swaine L; Ng, Lai Guan; Ding, Jeak Ling; Chong, Shu Zhen; Prof Jeak Ling Ding; MEDICINE; DUKE-NUS OFFICE OF ACAD & CLINICAL DEVT; MICROBIOLOGY AND IMMUNOLOGY; DEAN'S OFFICE (DUKE-NUS MEDICAL SCHOOL); BIOLOGICAL SCIENCES; CHEMICAL & BIOMOLECULAR ENGINEERING
    Circulating Ly6Chi monocytes often undergo cellular death upon exhaustion of their antibacterial effector functions, which limits their capacity for subsequent macrophage differentiation. This shrouds the understanding on how the host replaces the tissue-resident macrophage niche effectively during bacterial invasion to avert infection morbidity. Here, we show that proliferating transitional premonocytes (TpMos), an immediate precursor of mature Ly6Chi monocytes (MatMos), were mobilized into the periphery in response to acute bacterial infection and sepsis. TpMos were less susceptible to apoptosis and served as the main source of macrophage replenishment when MatMos were vulnerable toward bacteria-induced cellular death. Furthermore, TpMo and its derived macrophages contributed to host defense by balancing the proinflammatory cytokine response of MatMos. Consequently, adoptive transfer of TpMos improved the survival outcome of lethal sepsis. Our findings hence highlight a protective role for TpMos during bacterial infections and their contribution toward monocyte-derived macrophage heterogeneity in distinct disease outcomes.
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    Publication
    Blomia tropicalis-Specific TCR Transgenic Th2 Cells Induce Inducible BALT and Severe Asthma in Mice by an IL-4/IL-13-Dependent Mechanism
    (AMER ASSOC IMMUNOLOGISTS, 2016-11-15) Chua, Yen Leong; Liong, Ka Hang; Huang, Chiung-Hui; Wong, Hok Sum; Zhou, Qian; Ler, Say Siong; Tang, Yafang; Low, Chin Pei; Koh, Hui Yu; Kuo, I-Chun; Zhang, Yongliang; Wong, WS Fred; Peh, Hong Yong; Lim, Hwee Ying; Ge, Moyar Qing; Haczku, Angela; Angeli, Veronique; MacAry, Paul A; Chua, Kaw Yan; Kemeny, David M; Assoc Prof Paul A MacAry; PHARMACOLOGY; MICROBIOLOGY AND IMMUNOLOGY; SAW SWEE HOCK SCHOOL OF PUBLIC HEALTH; PAEDIATRICS
    Previous studies have highlighted the importance of lung-draining lymph nodes in the respiratory allergic immune response, whereas the lung parenchymal immune system has been largely neglected. We describe a new in vivo model of respiratory sensitization to Blomia tropicalis, the principal asthma allergen in the tropics, in which the immune response is focused on the lung parenchyma by transfer of Th2 cells from a novel TCR transgenic mouse, specific for the major B. tropicalis allergen Blo t 5, that targets the lung rather than the draining lymph nodes. Transfer of highly polarized transgenic CD4 effector Th2 cells, termed BT-II, followed by repeated inhalation of Blo t 5 expands these cells in the lung >100-fold, and subsequent Blo t 5 challenge induced decreased body temperature, reduction in movement, and a fall in specific lung compliance unseen in conventional mouse asthma models following a physiological allergen challenge. These mice exhibit lung eosinophilia; smooth muscle cell, collagen, and goblet cell hyperplasia; hyper IgE syndrome; mucus plugging; and extensive inducible BALT. In addition, there is a fall in total lung volume and forced expiratory volume at 100 ms. These pathophysiological changes were substantially reduced and, in some cases, completely abolished by administration of neutralizing mAbs specific for IL-4 and IL-13 on weeks 1, 2, and 3. This IL-4/IL-13-dependent inducible BALT model will be useful for investigating the pathophysiological mechanisms that underlie asthma and the development of more effective drugs for treating severe asthma.