Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo

Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bmcl.2004.09.083

Title:	Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo
Authors:	Tan, L.-P. Lue, R.Y.P. Chen, G.Y.J. Yao, S.Q.
Keywords:	Intein Protein array Protein biotinylation Proteomics
Issue Date:	20-Dec-2004
Citation:	Tan, L.-P., Lue, R.Y.P., Chen, G.Y.J., Yao, S.Q. (2004-12-20). Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorganic and Medicinal Chemistry Letters 14 (24) : 6067-6070. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2004.09.083
Abstract:	One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds. © 2004 Elsevier Ltd. All rights reserved.
Source Title:	Bioorganic and Medicinal Chemistry Letters
URI:	http://scholarbank.nus.edu.sg/handle/10635/76357
ISSN:	0960894X
DOI:	10.1016/j.bmcl.2004.09.083
Appears in Collections:	Staff Publications

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