Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bmcl.2004.09.083
DC Field | Value | |
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dc.title | Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo | |
dc.contributor.author | Tan, L.-P. | |
dc.contributor.author | Lue, R.Y.P. | |
dc.contributor.author | Chen, G.Y.J. | |
dc.contributor.author | Yao, S.Q. | |
dc.date.accessioned | 2014-06-23T05:41:47Z | |
dc.date.available | 2014-06-23T05:41:47Z | |
dc.date.issued | 2004-12-20 | |
dc.identifier.citation | Tan, L.-P., Lue, R.Y.P., Chen, G.Y.J., Yao, S.Q. (2004-12-20). Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorganic and Medicinal Chemistry Letters 14 (24) : 6067-6070. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2004.09.083 | |
dc.identifier.issn | 0960894X | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/76357 | |
dc.description.abstract | One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds. © 2004 Elsevier Ltd. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bmcl.2004.09.083 | |
dc.source | Scopus | |
dc.subject | Intein | |
dc.subject | Protein array | |
dc.subject | Protein biotinylation | |
dc.subject | Proteomics | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.department | CHEMISTRY | |
dc.description.doi | 10.1016/j.bmcl.2004.09.083 | |
dc.description.sourcetitle | Bioorganic and Medicinal Chemistry Letters | |
dc.description.volume | 14 | |
dc.description.issue | 24 | |
dc.description.page | 6067-6070 | |
dc.description.coden | BMCLE | |
dc.identifier.isiut | 000225522700025 | |
Appears in Collections: | Staff Publications |
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