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Title: In vivo bioactivity of rhBMP-2 delivered with novel polyelectrolyte complexation shells assembled on an alginate microbead core template
Authors: Abbah, S.-A.
Liu, J.
Lam, R.W.M.
Goh, J.C.H. 
Wong, H.-K.
Keywords: Controlled-release
Core-shell structure
Polyelectrolyte complexes
Recombinant human bone morphogenetic protein-2 (rhBMP-2)
Spinal fusion
Issue Date: 10-Sep-2012
Source: Abbah, S.-A.,Liu, J.,Lam, R.W.M.,Goh, J.C.H.,Wong, H.-K. (2012-09-10). In vivo bioactivity of rhBMP-2 delivered with novel polyelectrolyte complexation shells assembled on an alginate microbead core template. Journal of Controlled Release 162 (2) : 364-372. ScholarBank@NUS Repository.
Abstract: Electrostatic interactions between polycations and polyanions are being explored to fabricate polyelectrolyte complexes (PEC) that could entrap and regulate the release of a wide range of biomolecules. Here, we report the in vivo application of PEC shells fabricated from three different polycations: poly-l-ornithine (PLO), poly-l-arginine (PLA) and DEAE-dextran (DEAE-D) to condense heparin on the surface of alginate microbeads and further control the delivery of recombinant human bone morphogenetic protein 2 (rhBMP-2) in spinal fusion application. We observed large differences in the behavior of PEC shells fabricated from the cationic polyamino acids (PLO and PLA) when compared to the cationic polysaccharide, DEAE-D. Whereas DEAE-D-based PEC shells eroded and released rhBMP-2 over 2 days in vitro, PLO- and PLA-based shells retained at least 60% of loaded rhBMP-2 after 3 weeks of incubation in phosphate-buffered saline. In vivo implantation in a rat model of posterolateral spinal fusion revealed robust bone formation in the PLO- and PLA-based PEC shell groups. This resulted in a significantly enhanced mechanical stability of the fused segments. However, bone induction and biomechanical stability of spine segments implanted with DEAE-D-based carriers were significantly inferior to both PLO- and PLA-based PEC shell groups (p < 0.01). From these results, we conclude that PEC shells incorporating native heparin could be used for growth factor delivery in functional bone tissue engineering application and that PLA- and PLO-based complexes could represent superior options to DEAE-D for loading and in vivo delivery of bioactive BMP-2 in this approach. © 2012 Elsevier B.V.
Source Title: Journal of Controlled Release
ISSN: 01683659
DOI: 10.1016/j.jconrel.2012.07.027
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