Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.jconrel.2012.07.027
DC Field | Value | |
---|---|---|
dc.title | In vivo bioactivity of rhBMP-2 delivered with novel polyelectrolyte complexation shells assembled on an alginate microbead core template | |
dc.contributor.author | Abbah, S.-A. | |
dc.contributor.author | Liu, J. | |
dc.contributor.author | Lam, R.W.M. | |
dc.contributor.author | Goh, J.C.H. | |
dc.contributor.author | Wong, H.-K. | |
dc.date.accessioned | 2014-06-17T09:44:31Z | |
dc.date.available | 2014-06-17T09:44:31Z | |
dc.date.issued | 2012-09-10 | |
dc.identifier.citation | Abbah, S.-A., Liu, J., Lam, R.W.M., Goh, J.C.H., Wong, H.-K. (2012-09-10). In vivo bioactivity of rhBMP-2 delivered with novel polyelectrolyte complexation shells assembled on an alginate microbead core template. Journal of Controlled Release 162 (2) : 364-372. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jconrel.2012.07.027 | |
dc.identifier.issn | 01683659 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/67105 | |
dc.description.abstract | Electrostatic interactions between polycations and polyanions are being explored to fabricate polyelectrolyte complexes (PEC) that could entrap and regulate the release of a wide range of biomolecules. Here, we report the in vivo application of PEC shells fabricated from three different polycations: poly-l-ornithine (PLO), poly-l-arginine (PLA) and DEAE-dextran (DEAE-D) to condense heparin on the surface of alginate microbeads and further control the delivery of recombinant human bone morphogenetic protein 2 (rhBMP-2) in spinal fusion application. We observed large differences in the behavior of PEC shells fabricated from the cationic polyamino acids (PLO and PLA) when compared to the cationic polysaccharide, DEAE-D. Whereas DEAE-D-based PEC shells eroded and released rhBMP-2 over 2 days in vitro, PLO- and PLA-based shells retained at least 60% of loaded rhBMP-2 after 3 weeks of incubation in phosphate-buffered saline. In vivo implantation in a rat model of posterolateral spinal fusion revealed robust bone formation in the PLO- and PLA-based PEC shell groups. This resulted in a significantly enhanced mechanical stability of the fused segments. However, bone induction and biomechanical stability of spine segments implanted with DEAE-D-based carriers were significantly inferior to both PLO- and PLA-based PEC shell groups (p < 0.01). From these results, we conclude that PEC shells incorporating native heparin could be used for growth factor delivery in functional bone tissue engineering application and that PLA- and PLO-based complexes could represent superior options to DEAE-D for loading and in vivo delivery of bioactive BMP-2 in this approach. © 2012 Elsevier B.V. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jconrel.2012.07.027 | |
dc.source | Scopus | |
dc.subject | Controlled-release | |
dc.subject | Core-shell structure | |
dc.subject | Polyelectrolyte complexes | |
dc.subject | Recombinant human bone morphogenetic protein-2 (rhBMP-2) | |
dc.subject | Spinal fusion | |
dc.type | Article | |
dc.contributor.department | BIOENGINEERING | |
dc.description.doi | 10.1016/j.jconrel.2012.07.027 | |
dc.description.sourcetitle | Journal of Controlled Release | |
dc.description.volume | 162 | |
dc.description.issue | 2 | |
dc.description.page | 364-372 | |
dc.description.coden | JCREE | |
dc.identifier.isiut | 000310506400013 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.