Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/42965
Title: INTERLEUKIN-6 RELEASE FROM T98G HUMAN GLIAL CELL LINE AS A PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN
Authors: TAY SUAN ANNABEL
Keywords: Chronic pain, Postherpetic Neuralgia, Osteoarthritis, Interleukin-6, Cerebrospinal fluid, NF-kB
Issue Date: 8-Jan-2013
Source: TAY SUAN ANNABEL (2013-01-08). INTERLEUKIN-6 RELEASE FROM T98G HUMAN GLIAL CELL LINE AS A PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN. ScholarBank@NUS Repository.
Abstract: This work focuses on pro-inflammatory cytokine release in a cell culture system as a potential marker for chronic pain. Cerebrospinal fluid (CSF) from post-herpetic neuralgia (PHN) and osteroarthritic patients was used to trigger T98G cell cultures. Interleukin (IL-6) release in chronic pain patient groups was significantly higher than that of pain-free controls, and in the patient groups whose treatment was ineffective. CSF samples collected pre-treatment also triggered higher IL-6 than post-treatment samples. Secondly, fractions in chronic pain patients¿ CSF that triggered IL-6 release in T98G cells were isolated. They would be subjected to future mass spectrometry analysis to identify protein-like substances in CSF which could be potential chronic pain markers. Lastly, western blot analysis to elucidate the IL-6 signaling pathway showed NF-¿B acting upstream of IL-6. This in vitro assay system quantifying chronic pain and the understanding of the complex mechanisms could contribute to the future management of unrelenting pain.
URI: http://scholarbank.nus.edu.sg/handle/10635/42965
Appears in Collections:Master's Theses (Open)

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