Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/42965
DC Field | Value | |
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dc.title | INTERLEUKIN-6 RELEASE FROM T98G HUMAN GLIAL CELL LINE AS A PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN | |
dc.contributor.author | TAY SUAN ANNABEL | |
dc.date.accessioned | 2013-07-16T18:00:13Z | |
dc.date.available | 2013-07-16T18:00:13Z | |
dc.date.issued | 2013-01-08 | |
dc.identifier.citation | TAY SUAN ANNABEL (2013-01-08). INTERLEUKIN-6 RELEASE FROM T98G HUMAN GLIAL CELL LINE AS A PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/42965 | |
dc.description.abstract | This work focuses on pro-inflammatory cytokine release in a cell culture system as a potential marker for chronic pain. Cerebrospinal fluid (CSF) from post-herpetic neuralgia (PHN) and osteroarthritic patients was used to trigger T98G cell cultures. Interleukin (IL-6) release in chronic pain patient groups was significantly higher than that of pain-free controls, and in the patient groups whose treatment was ineffective. CSF samples collected pre-treatment also triggered higher IL-6 than post-treatment samples. Secondly, fractions in chronic pain patients¿ CSF that triggered IL-6 release in T98G cells were isolated. They would be subjected to future mass spectrometry analysis to identify protein-like substances in CSF which could be potential chronic pain markers. Lastly, western blot analysis to elucidate the IL-6 signaling pathway showed NF-¿B acting upstream of IL-6. This in vitro assay system quantifying chronic pain and the understanding of the complex mechanisms could contribute to the future management of unrelenting pain. | |
dc.language.iso | en | |
dc.subject | Chronic pain, Postherpetic Neuralgia, Osteoarthritis, Interleukin-6, Cerebrospinal fluid, NF-kB | |
dc.type | Thesis | |
dc.contributor.department | ANAESTHESIA | |
dc.contributor.supervisor | LOW CHIAN MING | |
dc.contributor.supervisor | LIU HERN CHOON, EUGENE | |
dc.contributor.supervisor | LEE TAT LEANG | |
dc.description.degree | Master's | |
dc.description.degreeconferred | MASTER OF SCIENCE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Master's Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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TaySA.pdf | 1.46 MB | Adobe PDF | OPEN | None | View/Download |
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