Please use this identifier to cite or link to this item:
|Title:||Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus|
|Citation:||Lu, L., Manopo, I., Leung, B.P., Chng, H.H., Ling, A.E., Chee, L.L., Ooi, E.E., Chan, S.-W., Kwang, J. (2004-04). Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus. Journal of Clinical Microbiology 42 (4) : 1570-1576. ScholarBank@NUS Repository. https://doi.org/10.1128/JCM.42.4.1570-1576.2004|
|Abstract:||Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.|
|Source Title:||Journal of Clinical Microbiology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 10, 2018
WEB OF SCIENCETM
checked on Nov 7, 2017
checked on Nov 23, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.