Please use this identifier to cite or link to this item:
https://doi.org/10.1128/JCM.42.4.1570-1576.2004
DC Field | Value | |
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dc.title | Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus | |
dc.contributor.author | Lu, L. | |
dc.contributor.author | Manopo, I. | |
dc.contributor.author | Leung, B.P. | |
dc.contributor.author | Chng, H.H. | |
dc.contributor.author | Ling, A.E. | |
dc.contributor.author | Chee, L.L. | |
dc.contributor.author | Ooi, E.E. | |
dc.contributor.author | Chan, S.-W. | |
dc.contributor.author | Kwang, J. | |
dc.date.accessioned | 2016-11-28T10:19:52Z | |
dc.date.available | 2016-11-28T10:19:52Z | |
dc.date.issued | 2004-04 | |
dc.identifier.citation | Lu, L., Manopo, I., Leung, B.P., Chng, H.H., Ling, A.E., Chee, L.L., Ooi, E.E., Chan, S.-W., Kwang, J. (2004-04). Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus. Journal of Clinical Microbiology 42 (4) : 1570-1576. ScholarBank@NUS Repository. https://doi.org/10.1128/JCM.42.4.1570-1576.2004 | |
dc.identifier.issn | 00951137 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/131408 | |
dc.description.abstract | Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1128/JCM.42.4.1570-1576.2004 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.contributor.department | PAEDIATRICS | |
dc.contributor.department | MICROBIOLOGY | |
dc.contributor.department | MEDICINE | |
dc.description.doi | 10.1128/JCM.42.4.1570-1576.2004 | |
dc.description.sourcetitle | Journal of Clinical Microbiology | |
dc.description.volume | 42 | |
dc.description.issue | 4 | |
dc.description.page | 1570-1576 | |
dc.description.coden | JCMID | |
dc.identifier.isiut | 000220963000029 | |
Appears in Collections: | Staff Publications |
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