THE ROLE OF OSTEOGENIC INDUCTION IN BONE TISSUE ENGINEERING AND REGENERATION

Abstract

Mesenchymal stem cells (MSC) undergo efficient osteogenic differentiation under defined conditions, which underlies their potential in engineering bone grafts for critical-size bone defects. However, the influence of osteogenic induction on MSC’s in-vivo regenerative capacities is yet not clear. Accordingly, a systematic study was designed based on human MSC (hfMSC) loaded three-dimensional biomaterial scaffolds, and the influence of osteogenic induction on the cellular secretome and bone healing efficacy was investigated. The in-vitro evaluations showed that osteogenic induction not only triggered hfMSC differentiation, but also promoted the proangiogenic paracrine activity of the bone grafts (>1.5-fold). Furthermore, a clinically relevant rat bone defect model was created and implanted with induced cellular scaffolds, and the results confirmed that prolonged osteogenic induction led to improved post-transplant neo-vascularization and more robust bone repair (2-fold). In general, this