Please use this identifier to cite or link to this item: https://doi.org/10.1080/152873901753215966
Title: Critical role of reactive oxygen species formation in microcystin-induced cytoskeleton disruption in primary cultured hepatocytes
Authors: Ding, W.-X.
Shen, H.-M. 
Ong, C.-N. 
Issue Date: 23-Nov-2001
Citation: Ding, W.-X., Shen, H.-M., Ong, C.-N. (2001-11-23). Critical role of reactive oxygen species formation in microcystin-induced cytoskeleton disruption in primary cultured hepatocytes. Journal of Toxicology and Environmental Health - Part A 64 (6) : 507-519. ScholarBank@NUS Repository. https://doi.org/10.1080/152873901753215966
Abstract: Cyanobacteria (blue-green algae)-contaminated water is a worldwide public health problem. Microcystins are a group of liver-specific toxins generated by cyanobacteria. It is generally believed that the protein phosphorylation that leads to the disruption of intermediate filaments plays an important role in microcystin-induced hepatotoxicity. However, the mechanisms that contribute to the microcystin-induced alterations of microtubules and microfilaments are not fully understood. In the present study, the effects of microcystin-LR (M-LR), the most common microcystin, were examined on the organization of cellular microtubules and microfilaments in primary cultured rat hepatocytes. Our results indicate that M-LR initiated reactive oxygen species (ROS) formation followed by altering the cytoskeleton structures, which eventually led to significant LDH leakage. These effects were completely prevented by TEMPOL, a superoxide dismutase mimic, and also partially prevented by desferoxamine. These findings provide further evidence that ROS formation, especially superoxide radical, plays a crucial role in M-LR-induced disruption of cytoskeleton organization and consequent hepatotoxicity.
Source Title: Journal of Toxicology and Environmental Health - Part A
URI: http://scholarbank.nus.edu.sg/handle/10635/113422
ISSN: 15287394
DOI: 10.1080/152873901753215966
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

119
checked on Sep 21, 2018

WEB OF SCIENCETM
Citations

116
checked on Sep 11, 2018

Page view(s)

24
checked on Jul 6, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.