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https://doi.org/10.1080/152873901753215966
DC Field | Value | |
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dc.title | Critical role of reactive oxygen species formation in microcystin-induced cytoskeleton disruption in primary cultured hepatocytes | |
dc.contributor.author | Ding, W.-X. | |
dc.contributor.author | Shen, H.-M. | |
dc.contributor.author | Ong, C.-N. | |
dc.date.accessioned | 2014-12-01T06:54:17Z | |
dc.date.available | 2014-12-01T06:54:17Z | |
dc.date.issued | 2001-11-23 | |
dc.identifier.citation | Ding, W.-X., Shen, H.-M., Ong, C.-N. (2001-11-23). Critical role of reactive oxygen species formation in microcystin-induced cytoskeleton disruption in primary cultured hepatocytes. Journal of Toxicology and Environmental Health - Part A 64 (6) : 507-519. ScholarBank@NUS Repository. https://doi.org/10.1080/152873901753215966 | |
dc.identifier.issn | 15287394 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/113422 | |
dc.description.abstract | Cyanobacteria (blue-green algae)-contaminated water is a worldwide public health problem. Microcystins are a group of liver-specific toxins generated by cyanobacteria. It is generally believed that the protein phosphorylation that leads to the disruption of intermediate filaments plays an important role in microcystin-induced hepatotoxicity. However, the mechanisms that contribute to the microcystin-induced alterations of microtubules and microfilaments are not fully understood. In the present study, the effects of microcystin-LR (M-LR), the most common microcystin, were examined on the organization of cellular microtubules and microfilaments in primary cultured rat hepatocytes. Our results indicate that M-LR initiated reactive oxygen species (ROS) formation followed by altering the cytoskeleton structures, which eventually led to significant LDH leakage. These effects were completely prevented by TEMPOL, a superoxide dismutase mimic, and also partially prevented by desferoxamine. These findings provide further evidence that ROS formation, especially superoxide radical, plays a crucial role in M-LR-induced disruption of cytoskeleton organization and consequent hepatotoxicity. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1080/152873901753215966 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | COMMUNITY,OCCUPATIONAL & FAMILY MEDICINE | |
dc.description.doi | 10.1080/152873901753215966 | |
dc.description.sourcetitle | Journal of Toxicology and Environmental Health - Part A | |
dc.description.volume | 64 | |
dc.description.issue | 6 | |
dc.description.page | 507-519 | |
dc.description.coden | JTEHD | |
dc.identifier.isiut | 000172378200007 | |
Appears in Collections: | Staff Publications |
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