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|Title:||Up-regulation of β1-adrenoceptor messenger ribonucleic acid in the rat pineal gland: Nocturnally, through a β-adrenoceptor-linked mechanism, and in vitro, through a novel posttranscriptional mechanism activated by specfic protein synthesis inhibitors|
|Citation:||Carter, D.A. (1993-11). Up-regulation of β1-adrenoceptor messenger ribonucleic acid in the rat pineal gland: Nocturnally, through a β-adrenoceptor-linked mechanism, and in vitro, through a novel posttranscriptional mechanism activated by specfic protein synthesis inhibitors. Endocrinology 133 (5) : 2263-2268. ScholarBank@NUS Repository.|
|Abstract:||The uniquely high concentration of β,-adrenoceptor mRNA (β1-mRNA) in the pineal gland provides a model for the regulation of GTP-binding protein (G-protein)-linked receptor gene expression within a functioning endocrine gland. By Northern analysis it has been shown that a nocturnal up-regulation of β1-mRNA in the rat pineal results in a 2.6-fold increase in mRNA levels at the middark phase (2400 h) compared with those at the midlight phase (1200 h). This increase is blocked by administration of the β-adrenoceptor antagonist propranolol before the onset of darkness. In vitro studies of β1-mRNA expression in organ-cultured pineals has confirmed β-adrenoceptorlinked up-regulation of β1-mRNA. Treatment of cultured pineals with the second messenger drugs forskolin and phorbol 12,13-dibutyrate produced mRNA responses that were again consistent with a primary role of β-adrenoceptors in the up-regulation of β1-mRNA. Nuclear runon analysis showed that the acute up-regulation of mRNA was mediated largely through a transcriptional mechanism. An additional novel mode of regulation of β-mRNA was also identified; the protein synthesis inhibitors anisomycin and cycloheximide, but not puromycin and emetine, elicited an acute increase in β1-mRNA in cultured pineals that was not transcriptionally mediated. The mechanism underlying this mode of regulation is discussed with relation to control of the cellular immediate early gene c-fos. The facility to examine both physiological and in vitro changes in β1-mRNA expression in the pineal will provide further insight into the complexity that is apparent for the molecular regulation of G-protein-coupled receptors.|
|Appears in Collections:||Staff Publications|
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