Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/112154
DC Field | Value | |
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dc.title | Up-regulation of β1-adrenoceptor messenger ribonucleic acid in the rat pineal gland: Nocturnally, through a β-adrenoceptor-linked mechanism, and in vitro, through a novel posttranscriptional mechanism activated by specfic protein synthesis inhibitors | |
dc.contributor.author | Carter, D.A. | |
dc.date.accessioned | 2014-11-28T02:53:48Z | |
dc.date.available | 2014-11-28T02:53:48Z | |
dc.date.issued | 1993-11 | |
dc.identifier.citation | Carter, D.A. (1993-11). Up-regulation of β1-adrenoceptor messenger ribonucleic acid in the rat pineal gland: Nocturnally, through a β-adrenoceptor-linked mechanism, and in vitro, through a novel posttranscriptional mechanism activated by specfic protein synthesis inhibitors. Endocrinology 133 (5) : 2263-2268. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00137227 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/112154 | |
dc.description.abstract | The uniquely high concentration of β,-adrenoceptor mRNA (β1-mRNA) in the pineal gland provides a model for the regulation of GTP-binding protein (G-protein)-linked receptor gene expression within a functioning endocrine gland. By Northern analysis it has been shown that a nocturnal up-regulation of β1-mRNA in the rat pineal results in a 2.6-fold increase in mRNA levels at the middark phase (2400 h) compared with those at the midlight phase (1200 h). This increase is blocked by administration of the β-adrenoceptor antagonist propranolol before the onset of darkness. In vitro studies of β1-mRNA expression in organ-cultured pineals has confirmed β-adrenoceptorlinked up-regulation of β1-mRNA. Treatment of cultured pineals with the second messenger drugs forskolin and phorbol 12,13-dibutyrate produced mRNA responses that were again consistent with a primary role of β-adrenoceptors in the up-regulation of β1-mRNA. Nuclear runon analysis showed that the acute up-regulation of mRNA was mediated largely through a transcriptional mechanism. An additional novel mode of regulation of β-mRNA was also identified; the protein synthesis inhibitors anisomycin and cycloheximide, but not puromycin and emetine, elicited an acute increase in β1-mRNA in cultured pineals that was not transcriptionally mediated. The mechanism underlying this mode of regulation is discussed with relation to control of the cellular immediate early gene c-fos. The facility to examine both physiological and in vitro changes in β1-mRNA expression in the pineal will provide further insight into the complexity that is apparent for the molecular regulation of G-protein-coupled receptors. | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.sourcetitle | Endocrinology | |
dc.description.volume | 133 | |
dc.description.issue | 5 | |
dc.description.page | 2263-2268 | |
dc.description.coden | ENDOA | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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