Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0068744
Title: MicroRNA-224 Targets SMAD Family Member 4 to Promote Cell Proliferation and Negatively Influence Patient Survival
Authors: Wang, Y.
Ren, J.
Gao, Y.
Ma, J.Z.I.
Toh, H.C.
Chow, P. 
Chung, A.Y.F.
Ooi, L.L.P.J.
Lee, C.G.L.
Issue Date: 29-Jul-2013
Citation: Wang, Y., Ren, J., Gao, Y., Ma, J.Z.I., Toh, H.C., Chow, P., Chung, A.Y.F., Ooi, L.L.P.J., Lee, C.G.L. (2013-07-29). MicroRNA-224 Targets SMAD Family Member 4 to Promote Cell Proliferation and Negatively Influence Patient Survival. PLoS ONE 8 (7) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0068744
Abstract: MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study, we further demonstrated that miR-224 up-regulation promotes cell proliferation using both in vitro assays and in vivo tumor growth models. We systematically screened for high confidence miR-224 targets by overlapping in silico predicted targets from multiple algorithms and significantly down-regulated genes in miR-224-expressing cells from whole genome expression microarrays. A total of 72 high confidence miR-224 targets were identified and found to be enriched in various cancer-related processes. SMAD family member 4 (SMAD4) is experimentally validated as the direct cellular target through which miR-224 promotes cell proliferation. The clinical relevance of our experimental observations was supported by a statistically significant inverse correlation between miR-224 and SMAD4 transcript expression in tumor versus paired adjacent non-tumorous tissues from HCC patients (p
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/110583
ISSN: 19326203
DOI: 10.1371/journal.pone.0068744
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