Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0068744
DC Field | Value | |
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dc.title | MicroRNA-224 Targets SMAD Family Member 4 to Promote Cell Proliferation and Negatively Influence Patient Survival | |
dc.contributor.author | Wang, Y. | |
dc.contributor.author | Ren, J. | |
dc.contributor.author | Gao, Y. | |
dc.contributor.author | Ma, J.Z.I. | |
dc.contributor.author | Toh, H.C. | |
dc.contributor.author | Chow, P. | |
dc.contributor.author | Chung, A.Y.F. | |
dc.contributor.author | Ooi, L.L.P.J. | |
dc.contributor.author | Lee, C.G.L. | |
dc.date.accessioned | 2014-11-26T09:04:40Z | |
dc.date.available | 2014-11-26T09:04:40Z | |
dc.date.issued | 2013-07-29 | |
dc.identifier.citation | Wang, Y., Ren, J., Gao, Y., Ma, J.Z.I., Toh, H.C., Chow, P., Chung, A.Y.F., Ooi, L.L.P.J., Lee, C.G.L. (2013-07-29). MicroRNA-224 Targets SMAD Family Member 4 to Promote Cell Proliferation and Negatively Influence Patient Survival. PLoS ONE 8 (7) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0068744 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/110583 | |
dc.description.abstract | MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study, we further demonstrated that miR-224 up-regulation promotes cell proliferation using both in vitro assays and in vivo tumor growth models. We systematically screened for high confidence miR-224 targets by overlapping in silico predicted targets from multiple algorithms and significantly down-regulated genes in miR-224-expressing cells from whole genome expression microarrays. A total of 72 high confidence miR-224 targets were identified and found to be enriched in various cancer-related processes. SMAD family member 4 (SMAD4) is experimentally validated as the direct cellular target through which miR-224 promotes cell proliferation. The clinical relevance of our experimental observations was supported by a statistically significant inverse correlation between miR-224 and SMAD4 transcript expression in tumor versus paired adjacent non-tumorous tissues from HCC patients (p | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0068744 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE | |
dc.description.doi | 10.1371/journal.pone.0068744 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 8 | |
dc.description.issue | 7 | |
dc.description.page | - | |
dc.description.coden | POLNC | |
dc.identifier.isiut | 000323369700011 | |
Appears in Collections: | Staff Publications Elements |
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2013-microRNA-224_targets_SMAD_family_member-pub.pdf | 1.43 MB | Adobe PDF | OPEN | Published | View/Download |
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