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|Title:||Production of monoclonal antibodies against human hepatocellular carcinoma by immunisation with a cell membrane preparation.|
|Citation:||Tan, L.S. (1990-03). Production of monoclonal antibodies against human hepatocellular carcinoma by immunisation with a cell membrane preparation.. Annals of the Academy of Medicine Singapore 19 (2) : 147-151. ScholarBank@NUS Repository.|
|Abstract:||Monoclonal antibodies that bound to 3 human hepatocellular carcinoma cell lines (PLC/PRF/5, SK-SF and Mahlavu) and snap-frozen sections of a patient's tumour were raised by immunising mice with PLC/PRF/5 membrane preparations. The monoclonals designated RF-HCC 1 and RF-HCC 2 were of IgG1 and IgM subclasses respectively. Binding of the antibodies to surface epitopes present on Mahlavu cells in vitro took place in a biphasic fashion, with initial rapid binding of each antibody during the first 15 minutes of incubation followed by a plateau after this period. It was found that the commercially available MOPC 21 myeloma IgG1 (Sigma) monoclonal antibody used a control also bound to a surface antigenic determinant expressed on Mahlavu cells.|
|Source Title:||Annals of the Academy of Medicine Singapore|
|Appears in Collections:||Staff Publications|
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