Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2012.04.056
DC FieldValue
dc.titleA β-sheet structure interacting peptide for intracellular protein delivery into human pluripotent stem cells and their derivatives
dc.contributor.authorLo, S.L.
dc.contributor.authorLua, S.
dc.contributor.authorSong, J.
dc.contributor.authorWang, S.
dc.date.accessioned2014-10-27T08:20:47Z
dc.date.available2014-10-27T08:20:47Z
dc.date.issued2012-05-11
dc.identifier.citationLo, S.L., Lua, S., Song, J., Wang, S. (2012-05-11). A β-sheet structure interacting peptide for intracellular protein delivery into human pluripotent stem cells and their derivatives. Biochemical and Biophysical Research Communications 421 (3) : 616-620. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2012.04.056
dc.identifier.issn0006291X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99990
dc.description.abstractThe advance in stem cell research relies largely on the efficiency and biocompatibility of technologies used to manipulate stem cells. In our previous study, we had designed an amphipathic peptide RV24 that can deliver proteins into cancer cell lines efficiently without significant side effects. Encouraged by this observation, we moved forward to test whether RV24 could be used to deliver proteins into human embryonic stem cells and human induced pluripotent stem cells. RV24 successfully mediated protein delivery into these pluripotent stem cells, as well as their derivatives including neural stem cells and dendritic cells. Based on NMR studies and particle surface charge measurements, we proposed that hydrophobic domain of RV24 interacts with β-sheet structures of the proteins, followed by formation of " peptide cage" to facilitate delivery across cellular membrane. These findings suggest the feasibility of using amphipathic peptide to deliver functional proteins intracellularly for stem cell research. © 2012 Elsevier Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2012.04.056
dc.sourceScopus
dc.subjectPeptide
dc.subjectPluripotent stem cells
dc.subjectProtein delivery
dc.subjectSelf-assembly
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.bbrc.2012.04.056
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume421
dc.description.issue3
dc.description.page616-620
dc.description.codenBBRCA
dc.identifier.isiut000306152000036
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

58
checked on May 24, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.