Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2004.05.004
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dc.titleA technique for preparing protein gradients on polymeric surfaces: Effects on PC12 pheochromocytoma cells
dc.contributor.authorLi, B.
dc.contributor.authorMa, Y.
dc.contributor.authorWang, S.
dc.contributor.authorMoran, P.M.
dc.date.accessioned2014-10-27T08:20:38Z
dc.date.available2014-10-27T08:20:38Z
dc.date.issued2005-05
dc.identifier.citationLi, B., Ma, Y., Wang, S., Moran, P.M. (2005-05). A technique for preparing protein gradients on polymeric surfaces: Effects on PC12 pheochromocytoma cells. Biomaterials 26 (13) : 1487-1495. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2004.05.004
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99976
dc.description.abstractA technique for preparing micropatterns and gradients of proteins on polymeric substrates has been developed in this work. Peroxides were generated on the substrate surface by UV preirradiation and they initiated graft polymerization of acrylic acid (AA) onto the surface upon a second UV irradiation. Micropatterns and gradients of poly(acrylic acid) (PAA) were formed when the substrate was placed under or moved with respect to a photomask during UV preirradiation. Protein micropatterns and gradients were fabricated on the surface by covalently linking to the carboxyl groups on PAA chains. To test cell response to the protein gradient surfaces, PC12 pheochromocytoma cells were cultured on laminin-bound substrates in serum-free medium supplemented with nerve growth factor (NGF). It is found that both the attachment and neurite outgrowth behaviors of PC12 cells were dependent on the surface laminin density. However, the unreacted carboxyl groups on the polymer surface negatively affected PC12 cells. This weakened the positive influence from laminin. © 2004 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2004.05.004
dc.sourceScopus
dc.subjectAcrylic acid
dc.subjectGraft polymerization
dc.subjectLaminin
dc.subjectMicropatterning
dc.subjectPC12 pheochromocytoma cells
dc.subjectProtein gradient
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.biomaterials.2004.05.004
dc.description.sourcetitleBiomaterials
dc.description.volume26
dc.description.issue13
dc.description.page1487-1495
dc.description.codenBIMAD
dc.identifier.isiut000226422200005
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