Please use this identifier to cite or link to this item:
https://doi.org/10.1021/ja053539b
| DC Field | Value | |
|---|---|---|
| dc.title | A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C, 15N-labeled large proteins | |
| dc.contributor.author | Xu, Y. | |
| dc.contributor.author | Lin, Z. | |
| dc.contributor.author | Ho, C. | |
| dc.contributor.author | Yang, D. | |
| dc.date.accessioned | 2014-10-27T08:19:07Z | |
| dc.date.available | 2014-10-27T08:19:07Z | |
| dc.date.issued | 2005-08-31 | |
| dc.identifier.citation | Xu, Y., Lin, Z., Ho, C., Yang, D. (2005-08-31). A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C, 15N-labeled large proteins. Journal of the American Chemical Society 127 (34) : 11920-11921. ScholarBank@NUS Repository. https://doi.org/10.1021/ja053539b | |
| dc.identifier.issn | 00027863 | |
| dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/99835 | |
| dc.description.abstract | A general strategy is proposed to assign aliphatic side-chain resonances of large 13C,15N-labeled proteins without deuteration, using 4D 13C,15N-edited NOESY and MQ-(H)CCH-TOCSY experiments on the basis of prior assignments of backbone and 13Cβ resonances. The strategy has been tested on a 214 residue protein (DdCAD-1) and applied to a chain-selectively 13C,15N-labeled hemoglobin (65 kDa). About 96 and 80% aliphatic side-chain spins in DdCAD-1 and hemoglobin have been assigned, respectively. The strategy proposed here will be very useful for the structure determination and dynamics characterization of large proteins by NMR. Copyright © 2005 American Chemical Society. | |
| dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/ja053539b | |
| dc.source | Scopus | |
| dc.type | Article | |
| dc.contributor.department | BIOLOGICAL SCIENCES | |
| dc.description.doi | 10.1021/ja053539b | |
| dc.description.sourcetitle | Journal of the American Chemical Society | |
| dc.description.volume | 127 | |
| dc.description.issue | 34 | |
| dc.description.page | 11920-11921 | |
| dc.description.coden | JACSA | |
| dc.identifier.isiut | 000231605900021 | |
| Appears in Collections: | Staff Publications | |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.