Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.virusres.2013.04.016
Title: A DNA-binding protein encoded by ORF008L of Singapore grouper iridovirus
Authors: Wang, L.
Chong, Q.Y.
Wu, J. 
Keywords: Collagen
DNA-binding protein
SGIV
Singapore grouper iridovirus
Issue Date: Sep-2013
Citation: Wang, L., Chong, Q.Y., Wu, J. (2013-09). A DNA-binding protein encoded by ORF008L of Singapore grouper iridovirus. Virus Research 176 (1-2) : 37-44. ScholarBank@NUS Repository. https://doi.org/10.1016/j.virusres.2013.04.016
Abstract: Singapore grouper iridovirus (SGIV) is a major viral pathogen that can cause substantial economic losses in aquaculture, but its genome replication, organization and package are largely unknown. We isolated SGIV protein-DNA core by freeze-thaw lysis of viral particles and gradient centrifugation. Twelve proteins were identified from the core by mass spectrometry. ORF008L, one of the core proteins, was identified as a collagen-like protein and its DNA binding ability was demonstrated by electrophoretic mobility shift assay (EMSA). Binding of ORF008L to DNA was neither sequence specific nor pH dependent, and it protected DNA from degradation by DNase I in vitro. These results suggest that ORF008L may play a role in protection or stabilization of the viral genome during infection. © 2013 Elsevier B.V.
Source Title: Virus Research
URI: http://scholarbank.nus.edu.sg/handle/10635/99828
ISSN: 01681702
DOI: 10.1016/j.virusres.2013.04.016
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