A DNA-binding protein encoded by ORF008L of Singapore grouper iridovirus

Abstract

Singapore grouper iridovirus (SGIV) is a major viral pathogen that can cause substantial economic losses in aquaculture, but its genome replication, organization and package are largely unknown. We isolated SGIV protein-DNA core by freeze-thaw lysis of viral particles and gradient centrifugation. Twelve proteins were identified from the core by mass spectrometry. ORF008L, one of the core proteins, was identified as a collagen-like protein and its DNA binding ability was demonstrated by electrophoretic mobility shift assay (EMSA). Binding of ORF008L to DNA was neither sequence specific nor pH dependent, and it protected DNA from degradation by DNase I in vitro. These results suggest that ORF008L may play a role in protection or stabilization of the viral genome during infection. © 2013 Elsevier B.V.