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Please use this identifier to cite or link to this item: https://doi.org/10.1042/BSR20130098
DC FieldValue
dc.titleA comparison and optimization of methods and factors affecting the transformation of Escherichia coli
dc.contributor.authorChan, W.-T.
dc.contributor.authorVerma, C.S.
dc.contributor.authorLane, D.P.
dc.contributor.authorGan, S.K.-E.
dc.date.accessioned2014-10-27T08:18:51Z
dc.date.available2014-10-27T08:18:51Z
dc.date.issued2013
dc.identifier.citationChan, W.-T., Verma, C.S., Lane, D.P., Gan, S.K.-E. (2013). A comparison and optimization of methods and factors affecting the transformation of Escherichia coli. Bioscience Reports 33 (6) : -. ScholarBank@NUS Repository. https://doi.org/10.1042/BSR20130098
dc.identifier.issn01448463
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99811
dc.description.abstractDNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2-CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-1 Blue, SCS110, JM109, TOP10 and BL21-(DE3)-PLysS. Hanahan's method was found to be most effective for DH5α, XL-1 Blue and JM109 strains (P
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1042/BSR20130098
dc.sourceScopus
dc.subjectCaCl2 method
dc.subjectCompetent bacteria
dc.subjectDMSO method
dc.subjectHanahan's method
dc.subjectHeat shock
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1042/BSR20130098
dc.description.sourcetitleBioscience Reports
dc.description.volume33
dc.description.issue6
dc.description.page-
dc.description.codenBRPTD
dc.identifier.isiut000329446200008
Appears in Collections:Staff Publications

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